The complete nucleotide sequence of VP5 of a strain of bluetongue virus of serotype 2 isolated in the USA reveals its close relationship with a virus of serotype 1 isolated in Australia.
The complete nucleotide sequence of VP5 of a strain of bluetongue virus of serotype 2 isolated in the USA reveals its close relationship with a virus of serotype 1 isolated in Australia.
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美国分离的一株血清型2蓝舌病病毒VP5的完整核苷酸序列揭示了其与澳大利亚分离的一种血清型1病毒的密切关系。
DOI：
10.1016/0168-1702(90)90002-s
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发表时间：
1990
期刊：
影响因子：
5
通讯作者：
Roy,P
中科院分区：
文献类型：
--
作者：
Hirasawa,T;Roy,P
蓝舌病毒2型（BTV-2）的M5基因长度为1635个碱基对，比美国BTV-10的M5基因短3个核苷酸，但与南非和澳大利亚的血清1型相同。由cDNA编码的氨基酸序列产生的526个氨基酸的分子大小为58 954道尔顿和短的非编码区的蛋白质。 在中性pH下，蛋白质的电荷为+1.5，而BTV-10的电荷为-4.5。BTV-1 SAfr. Aus、BTV-2和BTV-10使用BTV-10序列作为参考。BTV-1 Aus和BTV-2 U.S.之间的同源性高达96%;两种分子在相同的位置含有三个半胱氨酸残基。类似地，BTV-1 SAfr.而BTV-2美国只有10%。
The M5 RNA of bluetongue-virus-2 (BTV-2) was deduced from sequence analyses to be 1635 base pairs long, three nucleotides shorter than the M5 gene of BTV-10 U.S. but the same as serotype 1 (South Africa and Australia). The amino acid sequence encoded by the cDNA yielded a protein of 526 amino acids of molecular size 58 954 daltons and short non-coding regions. The charge of the protein is +1.5 at neutral pH compared with -4.5 of BTV-10. The amino acid sequence comparisons of BTV-1 SAfr. and Aus, BTV-2 and BTV-10 used the BTV-10 sequence as a reference. The homology between BTV-1 Aus and BTV-2 U.S. is up to 96%; both molecules contain three cysteine residues at the same positions. Similarly the variability in VP5 between BTV-1 SAfr. and BTV-2 U.S. is only 10%.