Synthetic peptides corresponding to sequences in HIV envelope gp41 and gp120 enhance in vitro production of interleukin-1 and tumor necrosis factor but depress production of interferon-alpha, interferon-gamma and interleukin-2.
Synthetic peptides corresponding to sequences in HIV envelope gp41 and gp120 enhance in vitro production of interleukin-1 and tumor necrosis factor but depress production of interferon-alpha, interferon-gamma and interleukin-2.
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与 HIV 包膜 gp41 和 gp120 中的序列相对应的合成肽可增强白细胞介素 1 和肿瘤坏死因子的体外产生，但抑制干扰素 α、干扰素 γ 和白细胞介素 2 的产生。
DOI：
10.1089/vim.1991.4.33
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发表时间：
1991
期刊：
影响因子：
2.2
通讯作者：
Kanda,P
中科院分区：
文献类型：
--
作者：
Tyring,SK;Cauda,R;Tumbarello,M;Ortona,L;Kennedy,RC;Chanh,TC;Kanda,P
人类免疫缺陷病毒（HIV）感染患者产生的淋巴因子和单核细胞因子的数量异常。包膜糖蛋白被认为在HIV发病机制中是重要的，并且可能影响这些细胞因子的产生。因此，研究了对应于糖蛋白gp 41的氨基酸序列735-752和846-860以及对应于gp 120的氨基酸序列304-328的合成肽在适当诱导剂存在下影响正常外周血单核细胞产生下列细胞因子的能力：干扰素（IFN）-α、IFN-γ、白细胞介素（IL）-1、IL-2和肿瘤坏死因子（TNF）。与单独的细胞和诱导剂（或在对照肽的存在下）相比，gp 41或gp 120合成肽能够抑制IFN-α、IFN-γ和IL-2的产生。相比之下，这些肽导致IL-1和TNF的产生增加。在大多数情况下，gp 41肽的效果比gp 120肽更显著。这些研究表明，这些HIV包膜糖蛋白可能是直接负责异常的淋巴因子和单核细胞因子的生产在感染这种病毒的患者，因此可能是至少部分负责艾滋病的发病机制。
Patients with human immunodeficiency virus (HIV) infections have aberrant production of a number of lymphokines and monokines. Envelope glycoproteins are believed to be important in HIV pathogenesis and may influence the production of these cytokines. Therefore, synthetic peptides corresponding to amino acid sequences 735–752 and 846–860 of glycoprotein gp41 and to amino acid sequence 304–328 of gp120 were investigated for their abilities to affect the production of the following cytokines by normal peripheral blood mononuclear cells in the presence of appropriate inducers: interferon (IFN)-α, IFN-γ, interleukin (IL)-1, IL-2, and tumor necrosis factor (TNF). In contrast to cells and inducers alone (or in the presence of a control peptide), gp41 or gp120 synthetic peptides were able to depress the production of IFN-α, IFN-γ and IL-2. In contrast, these peptides produced an elevation of the production of IL-1 and TNF. The effect of the gp41 peptides was more marked than that of gp120 peptides in most cases. These studies indicate that these HIV envelope glycoproteins may be directly responsible for aberrant lymphokine and monokine production in patients infected with this virus and therefore may be at least partially responsible for the pathogenesis of AIDS.