Lipid formation and gamma-linolenic acid production by Mucorales fungi grown on sunflower oil

Lipid formation and gamma-linolenic acid production by Mucorales fungi grown on sunflower oil
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DOI:
10.1046/j.1472-765x.1997.00173.x
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发表时间:
1997-08-01
影响因子:
2.4
通讯作者:
Sajbidor, J
Sajbidor, J
中科院分区:
生物学4区
文献类型:
--
作者:
Certik, M;Balteszova, L;Sajbidor, J

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研究了48种Mucorales真菌在葵花籽油(含70%亚油酸;LA)上生长的脂质形成和γ -亚麻酸(GLA)的产生。菌株生物量脂质积累量为42.7 ~ 65.8% (7.66 ~ 13.39 g l(-1))。8个培养物产生超过200 mg GLA l(-1)。GLA产量最高的是Mucor mucedo CCF-1384和Cunninghamella echinulata CCF-103(分别为379和373 mg l(-1))。高山孢杆菌CCF-185合成了465 mg l(-1)花生四烯酸。脂质利用指数(细胞脂质中脂质含量与油源中脂质含量之比)的降低伴随着脂质化生物量的增长和细胞内脂质积累的减少,而高脂质产量是由于油源直接掺入细胞内脂质的结果。
Lipid formation and gamma-linolenic acid (GLA) production by 48 species of Mucorales fungi grown on sunflower oil (which consists of 70% linoleic acid; LA) were studied. The strains accumulated 42.7-65.8% lipid in biomass (7.66-13.39 g l(-1)). Eight cultures produced more than 200 mg GLA l(-1). Highest GLA yields exhibited Mucor mucedo CCF-1384 and Cunninghamella echinulata CCF-103 (379 and 373 mg l(-1), respectively). Mortierella alpina CCF-185 synthesized 465 mg l(-1) arachidonic acid. While the decrease of LA utilization index (ratio of LA content of cell lipid/LA content of oil source) was accompanied with growth of delipidized biomass and with reduction of lipid accumulation within the cells, high lipid yield was as a consequence of the direct oil source incorporation into intracellular lipid.