Cell type-specific trans-activation by the B-myb gene product: requirement of the putative cofactor binding to the C-terminal conserved domain.
Cell type-specific trans-activation by the B-myb gene product: requirement of the putative cofactor binding to the C-terminal conserved domain.
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B-myb 基因产物的细胞类型特异性反式激活：需要推定的辅因子与 C 端保守结构域结合。
DOI：
10.1016/s0021-9258(18)33708-6
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发表时间：
1995
期刊：
影响因子：
8
通讯作者：
S. Ishii
中科院分区：
文献类型：
--
作者：
S. Tashiro;Y. Takemoto;H. Handa;S. Ishii
myb基因家族有三个成员，分别是c-myb、A-myb和B-myb。我们研究了B-myb基因产物（B-myb）在不同类型细胞中的反式激活能力。B-Myb在CV-1和HeLa细胞中起转录激活剂的作用，但在NIH3T3细胞中不起作用，这表明B-Myb是一种细胞类型特异性的转录激活剂。B-Myb的缺失分析表明，在myb基因家族的三个成员之间保守的区域（CR为保守区域）是B-Myb反式激活所必需的。一项体内竞争分析表明，与B-Myb的CR结合的调节因子(s)是交易激活所必需的。利用亲和树脂分析发现，有多种蛋白与B-Myb的CR结合，CV-1和HeLa细胞中的CR结合蛋白与NIH3T3细胞中的CR结合蛋白不同。这些结果表明，cr结合辅因子对B-Myb细胞类型特异性反式激活至关重要。
The myb gene family has three members, c-myb, A-myb and B-myb. We have examined the trans-activating capacity of the B-myb gene product (B-Myb) in various types of cells. B-Myb functions as a transcriptional activator in CV-1 and HeLa cells, but not in NIH3T3 cells, indicating that B-Myb is a cell type-specific transcriptional activator. Deletion analyses of B-Myb have demonstrated that the region conserved between three members of the myb gene family (CR for conserved region) is necessary for trans-activation by B-Myb. An in vivo competition assay suggests that regulatory factor(s) that binds to the CR of B-Myb is required for transactivation. Analyses using an affinity resin show that multiple proteins bind to the CR of B-Myb and that the CR-binding proteins in CV-1 and HeLa cells are different from those in NIH3T3 cells. These results suggest that the CR-binding cofactor(s) is critical for the cell type-specific trans-activation by B-Myb.