Embryonic stem cells and transgenic mice ubiquitously expressing a tau-tagged green fluorescent protein
Embryonic stem cells and transgenic mice ubiquitously expressing a tau-tagged green fluorescent protein
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DOI：
10.1006/dbio.2000.9935
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发表时间：
2000-12-01
影响因子：
2.7
通讯作者：
Mason, JO
中科院分区：
文献类型：
--
作者：
Pratt, T;Sharp, L;Mason, JO
我们已经产生了胚胎干（ES）细胞和转基因小鼠携带tau标记的绿色荧光蛋白（GFP）转基因的控制下，一个强大的启动子在所有类型的细胞，包括中枢神经系统的活动。GFP不需要底物，可以在固定或活细胞中检测到，因此是一种有吸引力的遗传标记。Tau标记的GFP通过将GFP结合到微管来标记亚细胞结构，包括轴突和有丝分裂机构。这使得细胞形态能够以精致的细节可视化。我们测试了来自这些小鼠的细胞在几种类型的细胞混合实验中的应用，并证明了表达tau-GFP的细胞的形态在它们整合到未标记的宿主细胞或组织中后可以很容易地可视化。我们预计，这些ES细胞和转基因小鼠将被证明是一种新的和强大的工具，为各种各样的应用，包括神经移植技术在动物模型和轴突寻路机制的基础研究的发展。tau-GFP标记的主要优点是它可以在活细胞和标记的细胞中被检测到，并且它们的过程可以被鉴定并进行各种操作，例如电生理学细胞记录。(C)北京大学出版社.
We have generated embryonic stem (ES) cells and transgenic mice carrying a tau-tagged green fluorescent protein (GFP) transgene under the control of a powerful promoter active in all cell types including those of the central nervous system. GFP requires no substrate and can be detected in fixed or living cells so is an attractive genetic marker. Tau-tagged GFP labels subcellular structures, including axons and the mitotic machinery, by binding the GFP to microtubules. This allows cell morphology to be visualized in exquisite detail. We test the application of cells derived from these mice in several types of cell-mixing experiments and demonstrate that the morphology of tau-GFP-expressing cells can be readily visualized after they have integrated into unlabeled host cells or tissues. We anticipate that these ES cells and transgenic mice will prove a novel and powerful tool for a wide variety of applications including the development of neural transplantation technologies in animal models and fundamental research into axon pathfinding mechanisms. A major advantage of the tau-GFP label is that it can be detected in living cells and labeled cells and their processes can be identified and subjected to a variety of manipulations such as electrophysiological cell recording. (C) 2000 Academic Press.