ESR spin trapping investigation on peroxynitrite decomposition: no evidence for hydroxyl radical production.
ESR spin trapping investigation on peroxynitrite decomposition: no evidence for hydroxyl radical production.
复制标题
过氧亚硝酸盐分解的 ESR 自旋捕获研究：没有羟基自由基产生的证据。
DOI：
--
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Y. Mao
中科院分区：
文献类型：
--
作者：
X. Shi;A. Lenhart;Y. Mao
过氧亚硝酸盐在 5,5-二甲基-1-吡咯啉 N-氧化物 (DMPO) 存在下分解生成 5,5-二甲基吡咯烷酮-(2)-氧基-(1) (DMPOX)，但不形成 DMPO/OH。甲酸盐增强了过氧亚硝酸盐的分解，但没有产生任何可检测量的甲酸盐衍生的自由基。谷胱甘肽、半胱氨酸、青霉胺和抗坏血酸盐与过氧亚硝酸盐反应生成相应的硫基和抗坏血酸自由基。结果表明，过亚硝酸盐的分解没有产生任何显着量的OH自由基，抗坏血酸对过亚硝酸盐的单电子还原可能是重要的过亚硝酸盐解毒途径之一。
The decomposition of peroxynitrite in the presence of 5,5-dimethyl-1-pyrroline N-oxide (DMPO) generated 5,5-dimethylpyrrolidone-(2)-oxy-(1) (DMPOX) without formation of DMPO/OH. Formate enhanced the peroxynitrite decomposition but did not generate any detectable amount of formate-derived free radicals. Glutathione, cysteine, penicillamine, and ascorbate reacted with peroxynitrite to generate the corresponding thiyl and ascorbyl radicals. The results show that the decomposition of peroxynitrite did not generate any significant amount of OH radicals, and one-electron reduction of peroxynitrite by ascorbate may be one of the important peroxynitrite detoxification pathways.