Protein Phosphatase Inhibition Assay for Detection of Microcystins in Lake Water and Microcystis Cultures
Protein Phosphatase Inhibition Assay for Detection of Microcystins in Lake Water and Microcystis Cultures
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用于检测湖水和微囊藻培养物中微囊藻毒素的蛋白磷酸酶抑制试验
DOI：
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Y. Takamura
中科院分区：
文献类型：
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作者：
Yao Yasuko;Y. Ujiie;Masayuki Watanabe;O. Yagi;Y. Takamura
采用蛋白磷酸酶抑制法测定了微囊藻水华污染湖水中低水平的微囊藻毒素含量和15株微囊藻培养物中的毒素含量。该方法灵敏度高，可测定μ g ~（1 -1）量级的微囊藻毒素。1995年夏季霞浦湖表层水中微囊藻毒素浓度为0.16 ~ 2.7μ g ~（1 -1），而M. aeruginosa TAC 192-2、M. ichthyoblabe TAC 69，M. ichthyoblabe TAC 113-1，M. viridis TAC 45-1、稻瘟病菌M. viridis TAC 64和M. 92. history of life同一种微囊藻的培养液中存在不产毒素的菌株。因此，培养液中的毒素产量不是鉴别微囊藻种的有用标准。
The protein phosphatase inhibition assay was used to quantify low levels of microcystin in lake water polluted by cyanobacterial blooms of Microcystis, and toxin concentrations in cultures of 15 strains of Microcystis. This method was highly sensitive permitting measurement of microcystin in concentrations of the order of μg1-1. The concentrations of microcystin in the surface water of Lake Kasumigaura in the summer of 1995 were in the range of 0.16 to 2.7μg1-1 microcystin-LR equivalents.On the other hand, markedly high levels of microcystin (160 to 1, 429μg1-1) were measured in the culture fluids of M. aeruginosa TAC 192-2, M. ichthyoblabe TAC 69, M. ichthyoblabe TAC 113-1, M. viridis TAC 45-1, M. viridis TAC 64 and M. viridis TAC 92. There were strains which did not produce microcystin in the culture fluid within the same species of Microcystis. Therefore, the toxin productivity in the culture fluid is not a useful criterion for discrimination of the species of Microcystis.
DOI：
10.1016/s0021-9258(17)45384-1
发表时间：
1990-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Honkanen;J. Zwiller;R. Moore;S. L. Daily;B. Khatra;M. Dukelow;A. Boynton
通讯作者：
R. Honkanen;J. Zwiller;R. Moore;S. L. Daily;B. Khatra;M. Dukelow;A. Boynton
影响因子：
2.8
作者：
AN, JS;CARMICHAEL, WW
通讯作者：
CARMICHAEL, WW