Identification of human T cell leukemia/lymphoma virus type I antibodies, DNA, and protein in patients with polymyositis.
Identification of human T cell leukemia/lymphoma virus type I antibodies, DNA, and protein in patients with polymyositis.
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多发性肌炎患者 T 细胞白血病/淋巴瘤病毒 I 型抗体、DNA 和蛋白质的鉴定。
DOI：
10.1002/art.1780380518
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发表时间：
1995
影响因子：
--
通讯作者：
GibbsJr,CJ
中科院分区：
文献类型：
--
作者：
Sherman,MP;Amin,RM;Rodgers-Johnson,PE;Morgan,OS;Char,G;Mora,CA;Iannone,R;Collins,GH;Papsidero,L;GibbsJr,CJ
目标。目的探讨人T细胞白血病/淋巴瘤病毒I型（HTLV‐I）与多发性肌炎（PM）之间的可能关系。将9名牙买加PM患者的血清和肌肉活检样本与美国HTLV‐i阳性PM患者和正常对照的样本进行比较。采用酶联免疫吸附试验和Western blot检测血清HTLV抗体。采用聚合酶链反应对活检样本进行HTLV‐I/II DNA分析，并对HTLV gp46包膜蛋白进行免疫组织化学染色。可获得血清的8例牙买加PM患者中有7例HTLV‐I血清阳性。9名牙买加患者的肌肉活检显示严重的淋巴细胞浸润、细胞变性、肌纤维萎缩和纤维化。每个肌肉活检标本含有HTLV‐I DNA。6个样本中的2个在许多入侵的单核细胞中显示HTLV‐I gp46的强烈染色，在许多入侵的单核细胞中显示HTLV‐I gp46的弱染色，在邻近的肌细胞中显示弱染色。另外两例罕见单核细胞gp46弱阳性。所有对照标本HTLV - I DNA和蛋白均为阴性。HTLV‐I与一种炎症性肌肉疾病有关，其特征是HTLV‐I感染的单核细胞直接侵袭受影响的肌肉。
Objective. To investigate a possible association between human T cell leukemia/lymphoma virus type I (HTLV‐I) and polymyositis (PM).Methods. Sera and muscle biopsy samples from 9 Jamaican PM patients were compared with specimens from American HTLV‐I–positive PM patients and normal controls. Sera were evaluated for HTLV antibodies by enzyme‐linked immunosorbent assay and Western blot. The biopsy samples were analyzed for HTLV‐I/II DNA by polymerase chain reaction and were also immunohistochemically stained for HTLV gp46 envelope protein.Results. Seven of the 8 Jamaican PM patients from whom sera were available were HTLV‐I seropositive. The muscle biopsies of all 9 Jamaican patients demonstrated severe lymphocytic infiltration, cellular degeneration, myofiber atrophy, and fibrosis. Each muscle biopsy specimen contained HTLV‐I DNA. Two of 6 samples demonstrated intense staining for HTLV‐I gp46 in many of the invading mononuclear cells and weak staining for HTLV‐I gp46 in many of the invading mononuclear cells and weak staining in the adjacent myocytes. Two other specimens were weakly positive for gp46 in rare mononuclear cells. All control specimens were negative for the presence of HTLV‐I DNA and protein.Conclusion. HTLV‐I is associated with an inflammatory muscle disease characterized by direct invasion of the affected muscle by HTLV‐I–infected mononuclear cells.
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1966
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DOI：
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DOI：
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期刊：
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