Effects of maternal undernutrition during late pregnancy on the development and function of ovine fetal liver
Effects of maternal undernutrition during late pregnancy on the development and function of ovine fetal liver
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DOI：
10.1016/j.anireprosci.2014.04.012
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发表时间：
2014-06-30
影响因子：
2.2
通讯作者：
Hou, Xianzhi
中科院分区：
文献类型：
--
作者：
Gao, Feng;Liu, Yingchun;Hou, Xianzhi
本研究探讨了妊娠后期营养不良对绵羊胎肝发育和功能的影响。在妊娠第90天，将18只单胎母羊分为3组：受限组1（RG 1，0.175 MJ ME kg BW-0.75 d（-1），n = 6），限制组2（RG 2，0.33 MJ ME kg BW-0.75 d（-1），n = 6）和对照组（CG，自由采食，0.67 MJ ME kg BW-0.75 d（-1），n = 6）。在第140天屠宰时回收胎仔。RG 1组胎仔的肝脏重量、总抗氧化能力（T-AOC）、超氧化物歧化酶（SOD）活性、胆碱酯酶（CHE）、总蛋白（TP）、球蛋白（GLB）和丙氨酸转氨酶（ALT）均降低（P < 0.05）。此外，RG 2组胎仔的肝脏重量、总抗氧化能力和胆碱酯酶（CHE）均下降（P < 0.05）。RG 1组胎肝胶原纤维和网状纤维、谷胱甘肽过氧化物酶（GSH-Px）、丙二醛（MDA）、一氧化氮（NO）、一氧化氮合酶（NOs）、单胺氧化酶（MAO）、白蛋白（ALB）/GLB、天冬氨酸转氨酶（AST）、AST/ALT升高（P < 0.05）。RG 2组胎肝胶原纤维、NO和MAO含量均高于对照组（P < 0.05）。这些结果表明，胎儿肝脏生长受损，纤维化，抗氧化失衡和功能障碍与母亲的不平衡。(C)2014爱思唯尔有限公司版权所有。
This study investigated the effects of maternal undernutrition during late pregnancy on the development and function of ovine fetal liver. Eighteen ewes with singleton fetuses were allocated to three groups at d 90 of pregnancy: Restricted Group 1 (RG1, 0.175 MJ ME kg BW-0.75 d(-1), n = 6), Restricted Group 2 (RG2, 0.33 MJ ME kg BW-0.75 d(-1), n = 6) and a Control Group (CG, ad libitum, 0.67 MJ ME kg BW-0.75 d(-1), n = 6). Fetuses were recovered at slaughter on d 140. Fetuses in the RG1 group exhibited decreased (P < 0.05) liver weight, total antioxidant capacity (T-AOC), superoxide dismutase activity (SOD), cholinesterase (CHE), total protein (TP), globulin (GLB), and alanine transaminase (ALT). In addition, intermediate changes were found in the RG2 fetuses, including decreased liver weight, T-AOC and CHE (P < 0.05). In contrast, increases in fetal hepatic collagen fibers and reticular fibers, glutathione peroxidase (GSH-Px), malondialdehyde (MDA), nitric oxide (NO), nitric oxide synthase (NOs), monoamine oxidase (MAO), albumin (ALB)/GLB, aspartate transaminase (AST), and AST/ALT were found in the RG1 fetuses (P < 0.05). The RG2 fetuses had increased fetal hepatic collagen fibers, NOs and MAO (P < 0.05) relative to the control fetuses. These results indicate that impaired fetal hepatic growth, fibrosis, antioxidant imbalance and dysfunction were associated with maternal undemutrition. (C) 2014 Elsevier B.V. All rights reserved.