ENHANCEMENT OF IN VIVO INCORPORATION OF LABELED PRECURSORS INTO DNA AND TOTAL PROTEIN OF MOUSE LYMPH NODES AFTER ADMINISTATION OF THYMIC EXTRACTS
ENHANCEMENT OF IN VIVO INCORPORATION OF LABELED PRECURSORS INTO DNA AND TOTAL PROTEIN OF MOUSE LYMPH NODES AFTER ADMINISTATION OF THYMIC EXTRACTS
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DOI：
10.1073/pnas.53.4.812
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发表时间：
1965-01-01
影响因子：
11.1
通讯作者：
WHITE, A
中科院分区：
文献类型：
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作者：
KLEIN, JJ;GOLDSTEIN, AL;WHITE, A
将小鼠、大鼠或小牛胸腺组织的无微粒氯化钠和酸性磷酸盐缓冲液浸提液注射至雄性CBA小鼠体内。24小时后，与接受盐水或缓冲液注射的对照组相比，注意到脉冲标记的H3-胸苷掺入外周淋巴结的核酸中显著增加。每天注射3次浸提液（72小时）后，观察到H3-胸苷掺入淋巴结的进一步增加。此时还观察到脉冲标记甘氨酸-2-C14掺入总蛋白的增加和淋巴结重量显著增加。牛血清白蛋白和大鼠淋巴结和脾脏提取物没有产生类似的效果。提示胸腺提取物对淋巴结增殖的影响是由于胸腺淋巴细胞生成因子的存在。所描述的测定方法应该是有用的，在化学特性的这个因素，并在其作用模式的研究。
Particle-free sodium chloride and acid phosphate buffer extracts of thymic tissue obtained from either mice, rats, or calves were injected into male CBA mice. After 24 hr, a significant increase was noted in pulse-label H3-thymidine incorporation into the nucleic acids of peripheral lymph nodes as compared to control groups receiving injections of saline or buffer. After 3 daily injections of extracts (72 hours) further increments in H3-thymidine incorporation into lymph nodes were observed. Increased pulse-label glycine-2-C14 incorporation into total protein and significantly increased lymph node weight also noted at this time. Bovine serum albumin and extracts of rat lymph nodes and spleen did not produce similar effects. It is suggested that the effects of the thymic extracts on lymph node proliferation are due to the presence of a thymic lymphocytopoietic factor. The assay method described should be useful in chemical characterization of this factor and in studies of its mode of action.