A behavioral comparison of the common laboratory rat strains Lister Hooded, Lewis, Fischer 344 and Wistar in an automated homecage system
A behavioral comparison of the common laboratory rat strains Lister Hooded, Lewis, Fischer 344 and Wistar in an automated homecage system
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DOI：
10.1111/gbb.12093
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发表时间：
2014-03-01
影响因子：
2.5
通讯作者：
Nguyen, H. P.
中科院分区：
文献类型：
--
作者：
Clemens, L. E.;Jansson, E. K. H.;Nguyen, H. P.
行为表征是建立新的动物模型的重要组成部分，但由于重复性和有效性方面的问题，经典的行为测试难以得出决定性的结果。相反，自动化的居家观察被认为可以产生与自然动物行为更相关的可靠结果。然而，关于背景菌株行为的信息很少，这可能会提供重要的参考材料。为此，我们比较了常用的Lister Hooded、Lewis、Fischer 344和Wistar大鼠在2、4和6个月龄的自动归巢系统中暴露70h的行为。我们发现，在代谢参数、新奇诱导和基线活动相关行为以及这些参数随年龄发展的差异方面，菌株之间存在相当大的差异。从系统与经典行为测试相比的优势和劣势，以及系统重建文献中常见发现的能力的角度对结果进行了讨论。
Behavioral characterization is an important part of establishing novel animal models, but classical behavioral tests struggle to reveal conclusive results due to problems with both reproducibility and validity. On the contrary, automated homecage observations are believed to produce robust outcomes that relate more to natural animal behavior. However, information on the behavior of background strains from such observations, which could provide important reference material, is rare. For this reason, we compared the behavior of the commonly used Lister Hooded, Lewis, Fischer 344 and Wistar rats during 70h of exposure to an automated homecage system at 2, 4 and 6months of age. We found considerable strain differences in metabolic parameters, novelty-induced and baseline activity-related behavior as well as differences in the development of these parameters with age. The results are discussed in terms of advantages and disadvantages of the system compared to classical behavioral tests, as well as the system's ability to recreate common findings in literature.