Apolipoprotein (apo) E inhibits the capacity of monosodium urate crystals to stimulate neutrophils. Characterization of intraarticular apo E and demonstration of apo E binding to urate crystals in vivo.
Apolipoprotein (apo) E inhibits the capacity of monosodium urate crystals to stimulate neutrophils. Characterization of intraarticular apo E and demonstration of apo E binding to urate crystals in vivo.
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DOI：
10.1172/jci114971
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发表时间：
1991
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影响因子：
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通讯作者：
Robert Terkeltaub;Cheryl A. Dyer;Jody L. Martin;Linda K. Curtiss
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文献类型：
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作者：
Robert Terkeltaub;Cheryl A. Dyer;Jody L. Martin;Linda K. Curtiss
调节尿酸单钠晶体刺激白细胞能力的因素可以调节痛风炎症。携带载脂蛋白B-100和载脂蛋白E的脂蛋白与尿酸盐晶体结合并抑制晶体与中性粒细胞的相互作用。在这项研究中，我们观察到包被单核细胞来源的载脂蛋白E的尿酸盐晶体刺激中性粒细胞的能力减弱。痛风患者尿酸盐结晶表面也检测到载脂蛋白E。因此，我们分析了非炎症性滑液中的载脂蛋白E，发现它与大小不一的颗粒有关，并且主要是阿尔法和β前电泳率。提示关节局部合成至少一部分apo E是因为(A)apo E的滑液/血浆浓度比明显高于apo B和apo A-I，而apo B和apo A-I不是由肝外组织广泛合成的，(B)第一代培养的类风湿滑膜细胞分泌apo E，(C)部分滑液中的apo E被严重分离。我们得出结论，滑液中含有部分起源于局部的载脂蛋白E。载脂蛋白E与尿酸盐晶体结合，可以调节痛风炎症。
Factors that modulate the ability of monosodium urate crystals to stimulate leukocytes could regulate gouty inflammation. Lipoproteins that bear apo B-100 and apo E bind to urate crystals and suppress crystal-neutrophil interaction. In this study, we observed that urate crystals, coated with apo E of monocyte origin, had a diminished ability to stimulate neutrophils. Apo E was also detected on the surface of urate crystals recovered from gout patients. Thus, we analyzed apo E in noninflammatory synovial fluid, and found it to be associated with particles of heterogeneous size and of predominantly alpha and pre-beta electrophoretic mobility. Local articular synthesis of at least a portion of synovial fluid apo E was suggested because (a) the synovial fluid/plasma concentration ratio of apo E was significantly higher than that for both apo B and apo A-I, which are not widely synthesized by extrahepatic tissues, (b) cultured rheumatoid synovial cells in first passage secreted apo E, (c) a portion of synovial fluid apo E was heavily sialylated. We conclude that synovial fluids contain apo E that appears partly of local origin. Apo E binds to urate crystals and could modulate gouty inflammation.