Effect of Shear Stress on Attachment of Corneal Endothelial Cells in Association With Corneal Endothelial Cell Loss After Laser Iridotomy
Effect of Shear Stress on Attachment of Corneal Endothelial Cells in Association With Corneal Endothelial Cell Loss After Laser Iridotomy
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DOI：
10.1097/01.ico.0000178735.27674.52
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发表时间：
2005-11
期刊：
影响因子：
2.8
通讯作者：
Y. Kaji;T. Oshika;T. Usui;J. Sakakibara
中科院分区：
文献类型：
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作者：
Y. Kaji;T. Oshika;T. Usui;J. Sakakibara
目的：激光虹膜切开术常引起大泡性角膜病变，其发生机制尚不清楚。我们研究了激光虹膜切开术后房水流体动力学的变化是否在角膜内皮细胞损失中有任何作用。材料和方法：将猪角膜内皮细胞接种于载玻片上。在粘附1或3小时后，将内皮细胞暴露于剪切应力（0.1-10达因/cm 2）15分钟，并计数脱离的细胞的数目。此外，在激光虹膜切开术的虚拟模型中计算角膜内皮层上的压力和剪切应力。结果：角膜内皮细胞脱落数随剪切力的增加而增加，并呈剂量依赖性。在粘附1小时后>0.3达因/厘米2和粘附3小时后1达因/厘米2时，观察到角膜内皮细胞脱落率显著增加。角膜内皮层的最大压力为0.007 mm Hg，与眼内压相比可以忽略不计。然而，在激光虹膜切开术的某些条件下，角膜内皮层上的最大剪切应力可>1达因/cm 2。结论：角膜内皮细胞对切应力的抵抗力随时间变化。在激光虹膜切开术的某些情况下，切应力可能是角膜内皮细胞损失的原因。
Purpose: Laser iridotomy often causes bullous keratopathy; however, the mechanism is unclear. We investigated whether changes in aqueous humor hydrodynamics after laser iridotomy have any role in corneal endothelial cell loss. Materials and Methods: Porcine corneal endothelial cells were plated onto glass slides. Following 1 or 3 hours for adhesion, the endothelial cells were exposed to shear stresses (0.1-10 dyne/cm2) for 15 minutes, and the number of detached cells was counted. In addition, the pressure and shear stress on corneal endothelial layer were calculated in a virtual model of laser iridotomy. Results: The number of detached corneal endothelial cells increased with shear stresses in a dose-dependent manner. Significant increase of rate of detached corneal endothelial cells was observed at >0.3 dyne/cm2 after 1-hour attachment and at 1 dyne/cm2 after 3-hour attachment. The maximum pressure on corneal endothelial layer was 0.007 mm Hg, which is negligible compared with intraocular pressure. However, the maximum shear stress on the corneal endothelial layer could be >1 dyne/cm2 in some conditions of laser iridotomy. Conclusions: The resistance of corneal endothelial cell loss to shear stress is time dependent. Shear stress could be a cause of corneal endothelial cell loss in some conditions of laser iridotomy.