Volumetric imaging of an intact organism by a distributed molecular network.
Volumetric imaging of an intact organism by a distributed molecular network.
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通过分布式分子网络对完整生物体进行体积成像。
DOI：
10.1101/2023.08.11.553025
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Weinstein,JoshuaA
中科院分区：
文献类型：
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作者：
Qian,Nianchao;Weinstein,JoshuaA
淋巴组织、神经组织和肿瘤组织等都表现出遗传独特细胞之间三维相互作用的生理学。因此，一种能够在一次从头测量中对转录组、基因型和形态进行体积成像的技术将为生命系统的生物复杂性提供一个批判性的视角。在这里，我们实现了这一点，通过扩展DNA显微镜，一种成像方式，编码一个标本的空间遗传图谱通过一个巨大的分布式网络的DNA分子在它里面，在发展中的斑马鱼胚胎的三维和多个长度尺度。
Lymphatic, nervous, and tumoral tissues, among others, exhibit physiology that emerges from three-dimensional interactions between genetically unique cells. A technology capable of volumetrically imaging transcriptomes, genotypes, and morphologies in a single de novo measurement would therefore provide a critical view into the biological complexity of living systems. Here we achieve this by extending DNA microscopy, an imaging modality that encodes a spatio-genetic map of a specimen via a massive distributed network of DNA molecules inside it, to three dimensions and multiple length scales in developing zebrafish embryos.