Photoacoustic imaging of lymphatic pumping
Photoacoustic imaging of lymphatic pumping
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DOI：
10.1117/1.jbo.22.10.106003
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发表时间：
2017-10-01
影响因子：
3.5
通讯作者：
Zemp, Roger J.
中科院分区：
文献类型：
--
作者：
Forbrich, Alex;Heinmiller, Andrew;Zemp, Roger J.
淋巴系统负责流体稳态和免疫细胞运输，并且已经涉及几种疾病，包括肥胖症、糖尿病和癌症转移。尽管其重要性，缺乏合适的体内成像技术阻碍了我们的淋巴系统的理解。这部分是由于淋巴液和结构的对比度有限。光声成像结合光吸收染料或纳米颗粒，具有很大的潜力，无创可视化淋巴管组织深处。多光谱光声成像能够分离组分;然而，大多数激光系统的慢波长切换速度不足以在不将运动伪影引入到处理的图像中的情况下对淋巴泵进行成像。我们研究两种方法可视化淋巴过程在体内。首先，单波长差分光声成像用于可视化淋巴泵在小鼠的后肢在真实的时间。第二，快速切换多波长光声成像系统被用来评估推进剖面的染料通过在真实的时间的双折射。这些方法可能对淋巴系统的非侵入性表征和研究产生深远的影响。(C)作者。由SPIE在知识共享署名3.0未移植许可下发布。
The lymphatic system is responsible for fluid homeostasis and immune cell trafficking and has been implicated in several diseases, including obesity, diabetes, and cancer metastasis. Despite its importance, the lack of suitable in vivo imaging techniques has hampered our understanding of the lymphatic system. This is, in part, due to the limited contrast of lymphatic fluids and structures. Photoacoustic imaging, in combination with optically absorbing dyes or nanoparticles, has great potential for noninvasively visualizing the lymphatic vessels deep in tissues. Multispectral photoacoustic imaging is capable of separating the components; however, the slow wavelength switching speed of most laser systems is inadequate for imaging lymphatic pumping without motion artifacts being introduced into the processed images. We investigate two approaches for visualizing lymphatic processes in vivo. First, single-wavelength differential photoacoustic imaging is used to visualize lymphatic pumping in the hindlimb of a mouse in real time. Second, a fast-switching multiwavelength photoacoustic imaging system was used to assess the propulsion profile of dyes through the lymphatics in real time. These approaches may have profound impacts in noninvasively characterizing and investigating the lymphatic system. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License.