A quantitative study of the division cycle of Caulobacter crescentus stalked cells
A quantitative study of the division cycle of Caulobacter crescentus stalked cells
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DOI：
10.1371/journal.pcbi.0040009.eor
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发表时间：
2008-01-01
影响因子：
4.3
通讯作者：
Tyson, John J.
中科院分区：
文献类型：
--
作者：
Li, Shenghua;Brazhnik, Paul;Tyson, John J.
细胞在分裂周期中的进程在不同的步骤被严格控制，以确保基因组复制和子细胞分裂的完整性。从已发表的实验证据中，我们提出了一种控制新月藻细胞分裂周期的分子机制。该机制基于三种“主调控蛋白”(CtrA、GcrA和Dna A)的合成和降解，并将其转化为定量模型，以研究这些蛋白和其他细胞周期蛋白的时间动力学。该模型解释了crescentus茎细胞的生理学、生化和细胞周期控制遗传学的重要细节。它在野生型细胞中复制蛋白质时间进程，正确模拟许多突变菌株的表型，并预测目前未确定特征的突变的表型。由于参与调节新月球藻细胞周期的许多蛋白质在许多α-变形杆菌属中都是保守的，所提出的机制可能适用于其他在农业和医学上具有重要意义的物种。
Progression of a cell through the division cycle is tightly controlled at different steps to ensure the integrity of genome replication and partitioning to daughter cells. From published experimental evidence, we propose a molecular mechanism for control of the cell division cycle in Caulobacter crescentus. The mechanism, which is based on the synthesis and degradation of three "master regulator'' proteins ( CtrA, GcrA, and DnaA), is converted into a quantitative model, in order to study the temporal dynamics of these and other cell cycle proteins. The model accounts for important details of the physiology, biochemistry, and genetics of cell cycle control in stalked C. crescentus cell. It reproduces protein time courses in wild-type cells, mimics correctly the phenotypes of many mutant strains, and predicts the phenotypes of currently uncharacterized mutants. Since many of the proteins involved in regulating the cell cycle of C. crescentus are conserved among many genera of alpha-proteobacteria, the proposed mechanism may be applicable to other species of importance in agriculture and medicine.