High speed, line-scanning, fiber bundle fluorescence confocal endomicroscopy for improved mosaicking.
High speed, line-scanning, fiber bundle fluorescence confocal endomicroscopy for improved mosaicking.
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DOI：
10.1364/boe.6.001241
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发表时间：
2015-04
影响因子：
3.4
通讯作者：
M. Hughes;Guang-Zhong Yang
中科院分区：
文献类型：
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作者：
M. Hughes;Guang-Zhong Yang
纤维束内窥镜系统的一个显着限制是视野往往很小，直径通常只有几百微米。图像镶嵌技术可以增加有效图像尺寸，但需要仔细操作探头以确保相邻帧之间有足够的重叠。对于帧速率通常为 10 fps 的共焦内窥镜来说，这是特别具有挑战性的。在本文中，我们证明，通过使用高速线性 CCD 相机，线扫描共焦内窥镜可以实现 120 fps 的帧速率，同时保持足够的分辨率和信噪比，以允许对局部染色的胃肠组织进行成像。与较低帧速率系统相比，这导致基于互相关的镶嵌算法的性能得到改善。
A significant limitation of fiber bundle endomicroscopy systems is that the field of view tends to be small, usually only several hundred micrometers in diameter. Image mosaicking techniques can increase the effective image size, but require careful manipulation of the probe to ensure sufficient overlap between adjacent frames. For confocal endomicroscopes, which typically have frame rates on the order of 10 fps, this is particularly challenging. In this paper we demonstrate that line-scanning confocal endomicroscopy can, by use of a high speed linear CCD camera, achieve a frame rate of 120 fps while maintaining sufficient resolution and signal-to-noise ratio to allow imaging of topically stained gastrointestinal tissues. This leads to improved performance of a cross-correlation based mosaicking algorithm when compared with lower frame-rate systems.