Characterizing Regulatory and Functional Differentiation between Maize Mesophyll and Bundle Sheath Cells by Transcriptomic Analysis
Characterizing Regulatory and Functional Differentiation between Maize Mesophyll and Bundle Sheath Cells by Transcriptomic Analysis
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DOI：
10.1104/pp.112.203810
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发表时间：
2012-09-01
期刊：
影响因子：
7.4
通讯作者：
Ku, Maurice S. B.
中科院分区：
文献类型：
--
作者：
Chang, Yao-Ming;Liu, Wen-Yu;Ku, Maurice S. B.
为了研究玉米叶肉细胞（M）和维管束鞘细胞（BS）之间的调控和功能分化，我们从新成熟的第二片叶中分离了大量高度同源的M和BS细胞，通过RNA测序进行转录组分析。在两个转录组中发现了总共52，421个具有至少一个读段的注释基因。定义一个基因，每百万个映射的读段中有一个以上的读段，我们确定了18，482个表达的基因; 14，972个在M细胞中表达，包括53个M富集的转录因子（TF）基因，而17，269个在BS细胞中表达，包括214个BS富集的TF基因。有趣的是，许多TF基因家族在表达中表现出明显的BS偏好。通路分析揭示了两种细胞类型在各种功能类别中的差异，M细胞在光反应，蛋白质合成和折叠，四吡咯合成和RNA结合中发挥更重要的作用，而BS细胞专门从事运输，信号传导，蛋白质降解和翻译后修饰，主要的碳，氢和氧代谢，细胞分裂和组织以及发育。据我们所知，编码参与C-4代谢物穿梭和BS细胞壁发育的几种转运蛋白的基因首次被鉴定。这一全面的数据集将有助于研究M/BS分化的调节和功能。
To study the regulatory and functional differentiation between the mesophyll (M) and bundle sheath (BS) cells of maize (Zea mays), we isolated large quantities of highly homogeneous M and BS cells from newly matured second leaves for transcriptome profiling by RNA sequencing. A total of 52,421 annotated genes with at least one read were found in the two transcriptomes. Defining a gene with more than one read per kilobase per million mapped reads as expressed, we identified 18,482 expressed genes; 14,972 were expressed in M cells, including 53 M-enriched transcription factor (TF) genes, whereas 17,269 were expressed in BS cells, including 214 BS-enriched TF genes. Interestingly, many TF gene families show a conspicuous BS preference in expression. Pathway analyses reveal differentiation between the two cell types in various functional categories, with the M cells playing more important roles in light reaction, protein synthesis and folding, tetrapyrrole synthesis, and RNA binding, while the BS cells specialize in transport, signaling, protein degradation and posttranslational modification, major carbon, hydrogen, and oxygen metabolism, cell division and organization, and development. Genes coding for several transporters involved in the shuttle of C-4 metabolites and BS cell wall development have been identified, to our knowledge, for the first time. This comprehensive data set will be useful for studying M/BS differentiation in regulation and function.