The ARC1 E3 Ligase Gene Is Frequently Deleted in Self-Compatible Brassicaceae Species and Has a Conserved Role in Arabidopsis lyrata Self-Pollen Rejection
The ARC1 E3 Ligase Gene Is Frequently Deleted in Self-Compatible Brassicaceae Species and Has a Conserved Role in Arabidopsis lyrata Self-Pollen Rejection
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DOI：
10.1105/tpc.112.104943
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发表时间：
2012-11-01
期刊：
影响因子：
11.6
通讯作者：
Goring, Daphne R.
中科院分区：
文献类型：
--
作者：
Indriolo, Emily;Tharmapalan, Pirashaanthy;Goring, Daphne R.
自花花粉排斥是有花植物重要的生殖调节因子，几种不同的细胞间信号系统已经进化到引发这种反应。在菊科植物中，自交不亲和系统是由花粉S位点Cys-Rich/S位点蛋白11（SCR/SP11）配体和雌蕊S受体激酶（SRK）介导的。虽然SCR/SP11-SRK识别系统已被确定在几个物种横跨菊科，鲜为人知的是，在柱头SRK激活的细胞反应的保护，自我花粉接触。ARMRepeatContaining 1（ARC 1）E3泛素连接酶在芸苔属植物中SRK下游发挥作用，参与自交不亲和反应，但有人认为ARC 1在拟南芥中不是必需的。在这里，我们调查了ARC 1直系同源物在几个最近测序的基因组中的存在，这些基因组来自于2000万至4000万年前已经多样化的菊科物种。令人惊讶的是，ARC 1基因在几个已经失去自交不亲和性状的物种中被删除，这表明ARC 1可能在向自交过渡的过程中失去功能。为了测试ARC 1在自交不亲和的拟南芥物种中的需求，产生转基因ARC 1 RNA干扰的拟南芥lyrata植物，并且它们表现出降低的自交不亲和反应，导致成功受精。因此，这项研究表明，ARC 1的自我花粉排斥反应内的菊科保守的作用。
Self-pollen rejection is an important reproductive regulator in flowering plants, and several different intercellular signaling systems have evolved to elicit this response. In the Brassicaceae, the self-incompatibility system is mediated by the pollen S-locus Cys-Rich/S-locus Protein11 (SCR/SP11) ligand and the pistil S Receptor Kinase (SRK). While the SCR/SP11-SRK recognition system has been identified in several species across the Brassicaceae, less is known about the conservation of the SRK-activated cellular responses in the stigma, following self-pollen contact. The ARM Repeat Containing1 (ARC1) E3 ubiquitin ligase functions downstream of SRK for the self-incompatibility response in Brassica, but it has been suggested that ARC1 is not required in Arabidopsis species. Here, we surveyed the presence of ARC1 orthologs in several recently sequenced genomes from Brassicaceae species that had diversified similar to 20 to 40 million years ago. Surprisingly, the ARC1 gene was deleted in several species that had lost the self-incompatibility trait, suggesting that ARC1 may lose functionality in the transition to self-mating. To test the requirement of ARC1 in a self-incompatible Arabidopsis species, transgenic ARC1 RNA interference Arabidopsis lyrata plants were generated, and they exhibited reduced self-incompatibility responses resulting in successful fertilization. Thus, this study demonstrates a conserved role for ARC1 in the self-pollen rejection response within the Brassicaceae.