Iron Biomineral Growth from the Initial Nucleation Seed in L-Ferritin
Iron Biomineral Growth from the Initial Nucleation Seed in L-Ferritin
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DOI：
10.1002/chem.202000064
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发表时间：
2020-03-31
影响因子：
4.3
通讯作者：
Turano, Paola
中科院分区：
文献类型：
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作者：
Ciambellotti, Silvia;Pozzi, Cecilia;Turano, Paola
通过在不同时间间隔冷冻铁盐暴露后的蛋白晶体，解析了均聚物人l -铁蛋白和马脾铁蛋白的x射线结构，发现在蛋白笼内表面生长了一个八核铁团簇，其中一些谷氨酸残基起关键作用。提供了从（mu(3)-oxo）tris[(mu(2)-glutamato-kappa O:kappa O')](glutamato-kappa O)(diaquo)triiron（III）种子开始形成团簇的原子分辨率视图。这些结果支持铁蛋白中的铁生物矿化是一个在蛋白质表面水平启动的过程，能够贡献配位键和静电引导。
X-ray structures of homopolymeric human L-ferritin and horse spleen ferritin were solved by freezing protein crystals at different time intervals after exposure to a ferric salt and revealed the growth of an octa-nuclear iron cluster on the inner surface of the protein cage with a key role played by some glutamate residues. An atomic resolution view of how the cluster formation develops starting from a (mu(3)-oxo)tris[(mu(2)-glutamato-kappa O:kappa O')](glutamato-kappa O)(diaquo)triiron(III) seed is provided. The results support the idea that iron biomineralization in ferritin is a process initiating at the level of the protein surface, capable of contributing coordination bonds and electrostatic guidance.