1H, 13C and 15N NMR backbone assignments of 25.5 kDa metallo-beta-lactamase from Bacteroides fragilis.
1H, 13C and 15N NMR backbone assignments of 25.5 kDa metallo-beta-lactamase from Bacteroides fragilis.
复制标题
来自脆弱拟杆菌的 25.5 kDa 金属-β-内酰胺酶的 1H、13C 和 15N NMR 主链归属。
DOI：
10.1023/a:1008279832041
复制
发表时间：
1998
影响因子：
2.7
通讯作者：
Dyson,HJ
中科院分区：
文献类型：
--
作者：
Scrofani,SD;Wright,PE;Dyson,HJ
Methods and resultsThe gene encoding for residues 18–249 of CcrA was expressed in Escherichia coli BL21 (DE3) cells (Yang et al., 1992) in a modified minimal media preparation. Metallo-β-lactamase preparations were assayed using nitrocefin (Oxoid), and yielded kinetic values consistent with previously published results (Yang et al., 1992).NMR spectra were acquired at 295 K on Bruker AMX-500,-600 and DRX-600 spectrometers equipped with triple resonance probes. All triple resonance experiments were performed on either 13C, 15N-or 2H, 13C, 15N-uniformly labeled CcrA. 15N-HSQC, 15N-edited TOCSY-HSQC and NOESY-HSQC experiments were performed on 15N-uniformly labeled CcrA. All samples were prepared in 10 mM HEPES
