Protein-Controlled Actuation of Dynamic Nucleic Acid Networks by Using Synthetic DNA Translators
Protein-Controlled Actuation of Dynamic Nucleic Acid Networks by Using Synthetic DNA Translators
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DOI：
10.1002/anie.202008553
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发表时间：
2020-09-02
影响因子：
16.6
通讯作者：
Ricci, Francesco
中科院分区：
文献类型：
--
作者：
Bertucci, Alessandro;Porchetta, Alessandro;Ricci, Francesco
将动态DNA纳米技术与蛋白质控制的驱动相结合，将扩大我们处理分子信息的能力。我们已经开发了一种策略，通过设计合成的DNA翻译器，通过编程的构象变化将特定的蛋白质结合事件转换为触发输入，从而使用DNA结合蛋白来启动链置换反应。我们已经构建了两种不同的DNA结合蛋白TATA结合蛋白和Myc-Max的合成DNA网络，并展示了链置换反应的多输入激活。我们通过基于DNA的人工通讯实现了对合成RNA和酶的蛋白质控制调节，显示了我们的分子系统在执行进一步可编程任务方面的潜力。
Integrating dynamic DNA nanotechnology with protein-controlled actuation will expand our ability to process molecular information. We have developed a strategy to actuate strand displacement reactions using DNA-binding proteins by engineering synthetic DNA translators that convert specific protein-binding events into trigger inputs through a programmed conformational change. We have constructed synthetic DNA networks responsive to two different DNA-binding proteins, TATA-binding protein and Myc-Max, and demonstrated multi-input activation of strand displacement reactions. We achieved protein-controlled regulation of a synthetic RNA and of an enzyme through artificial DNA-based communication, showing the potential of our molecular system in performing further programmable tasks.