Development of endodontic sealers containing antimicrobial-loaded polymer particles with long-term antibacterial effects
Development of endodontic sealers containing antimicrobial-loaded polymer particles with long-term antibacterial effects
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DOI：
10.1016/j.dental.2021.04.008
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发表时间：
2021-07-13
期刊：
影响因子：
5
通讯作者：
Imazato, Satoshi
中科院分区：
文献类型：
--
作者：
Kitagawa, Haruaki;Kitagawa, Ranna;Imazato, Satoshi
目标。本研究的目的是制备具有持久抗菌活性的新型牙用树脂。具体来说，本研究评估了一种控制根管感染的方法，该方法使用含有载有十六烷基吡啶氯（CPC）的聚甲基丙烯酸羟乙酯三甲基丙烯三甲基丙烯酸酯（polyHEMA/TMPT）颗粒的密封剂。此外，还测定了含有cpc负载的聚hema /TMPT颗粒（CLP）的密封剂的物理性能。制备了含有10% （10%-CLP）和25wt .% CPC （25%-CLP）的不同粒径的PolyHEMA/TMPT颗粒，并将其掺入hema基密封剂中。在水中浸泡14天，然后是14天的储存和14天的水中浸泡，评估了cpc的释放曲线。使用根管感染模型评估这些封口剂对牙本质小管内粪肠球菌的抗菌活性。通过流体过滤对其密封性能进行了评价，并根据ISO 6876标准对其物理性能进行了测试。含25%-CLP（类似于21 μ m颗粒直径）的固化封口剂在储存1年后重新评估其长期抗菌活性。浸泡28天后，25%-CLP比10%-CLP表现出更高和持续的CPC释放。采用含25% clp的封闭剂封闭根尖牙本质小管内的残留细菌。25%-CLP （21 μ m）的掺入对封口剂的密封能力和物理性能没有不良影响，并具有长期的抗菌活性。将负载cpc的颗粒掺入HEMA树脂中制备出具有长期杀菌活性的根管密封剂。这些封口剂可以潜在地用于预防复发的根尖牙周炎。(C) 2021牙科材料学会。Elsevier Inc.出版。版权所有。
Objective. The objective of this study is to prepare new dental resins with a long-lasting antimicrobial activity. Specifically, this study evaluates an approach for controlling infection in root canals using sealers containing polyhydroxyethyl methacrylate trimethylolpropane trimethacrylate (polyHEMA/TMPT) particles loaded with cetylpyridinium chloride (CPC). In addition, the physical properties of sealers containing CPC-loaded polyHEMA/TMPT particles (CLP) are determined.Methods. PolyHEMA/TMPT particles with 10 (10%-CLP) and 25 wt.% CPC (25%-CLP) with different particle sizes were fabricated and incorporated in HEMA-based sealers. CPC-release profiles were evaluated over 14 days of immersion in water, followed by 14 days of storage and 14 days of water immersion. The antibacterial activity of these sealers against Enterococcus faecalis in dentinal tubules was assessed using a root-canal-infection model. Their sealing abilities were evaluated by fluid filtration and physical properties were tested according to the ISO 6876 standard. The long-term antibacterial activity of the cured sealer containing 25%-CLP (similar to 21 mu m particle diameter) was re-assessed after 1 year of storage.Results. After 28 days of immersion, 25%-CLP exhibited a higher and sustained CPC release unlike 10%-CLP. Residual bacteria in root dentinal tubules were eradicated by obturation with 25%-CLP-containing sealers. The incorporation of 25%-CLP (similar to 21 mu m) had no adverse effects on the sealing ability and physical properties of the sealer and resulted in long-term antibacterial activity.Significance. The incorporation of CPC-loaded particles in HEMA resins yielded endodontic sealers with long-term bactericidal activity against E. faecalis in root canals. These sealers can potentially be used to prevent recurrent apical periodontitis. (C) 2021 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.