Quantitative Molecular Detection of 19 Major Pathogens in the Interdental Biofilm of Periodontally Healthy Young Adults.
Quantitative Molecular Detection of 19 Major Pathogens in the Interdental Biofilm of Periodontally Healthy Young Adults.
复制标题
DOI：
10.3389/fmicb.2016.00840
复制
发表时间：
2016
影响因子：
5.2
通讯作者：
Bourgeois D
中科院分区：
文献类型：
--
作者：
Carrouel F;Viennot S;Santamaria J;Veber P;Bourgeois D
在口腔健康中，牙间间隙是一个真正的生态位，身体几乎没有或没有替代的防御措施，而且传统的日常通过破坏生物膜来控制的方法是不够的。牙间间隙是许多关于它们与心血管疾病、糖尿病、慢性肾脏疾病、退行性疾病和抑郁症的潜在联系和/或原因的假说的来源。这项聚合酶链式反应研究首次参照苏格拉斯基复合体描述了18-35岁健康成年人的牙间微生物区系。这些复合体倾向于反映牙科生物膜形成过程中的微生物演替事件。早期的殖民者包括黄色、绿色和紫色复合体的成员。橙色复合菌一般出现在早期定殖者之后，并包括许多可能的牙周病原体，如核梭杆菌。红色复合体(牙龈卟啉单胞菌、连翘坦纳氏菌和齿密螺旋体)被认为是顶端群落，并被列入牙周病原体的推测名单。19种主要牙周病原菌均有不同程度的表达。种类最多的是核假单胞菌，其次是粘性放线菌、牙龈假单胞菌和伴生放线杆菌。腐蚀性艾肯氏杆菌、简约弯曲杆菌、直肠弯曲杆菌、齿状棘球绦虫和福氏唐纳氏杆菌的基因组数随着受试者年龄的增加而显著增加。这项研究强调指出，来自黄色复合体(链球菌、米蒂斯链球菌)、绿色复合体(腐蚀性链球菌、纤细弯曲菌、土黄色Capnocytophaga chracea、Capnocytophaga sputigena、伴生放线菌)、紫色复合体(微小韦洛氏菌、齿状放线菌)和蓝色复合体(粘性放线菌)的细菌是相关的。在橙色复合体方面，核心菌是牙间生物膜中含量最丰富的物种。红色复合体被认为是成人牙周病最重要的病原体，占所分析的19种细菌的8.08%。牙龈假单胞菌在19%的健康受试者中检出，占牙间生物膜的0.02%。在93%和49%的健康受试者中，分别检出齿间生物膜的0.02%和0.04%。牙周病原体的有效存在是一个强烈的迹象，表明需要开发新的方法来破坏日常口腔卫生中的牙间生物膜。
In oral health, the interdental spaces are a real ecological niche for which the body has few or no alternative defenses and where the traditional daily methods for control by disrupting biofilm are not adequate. The interdental spaces are the source of many hypotheses regarding their potential associations with and/or causes of cardiovascular disease, diabetes, chronic kidney disease, degenerative disease, and depression. This PCR study is the first to describe the interdental microbiota in healthy adults aged 18–35 years-old with reference to the Socransky complexes. The complexes tended to reflect microbial succession events in developing dental biofilms. Early colonizers included members of the yellow, green, and purple complexes. The orange complex bacteria generally appear after the early colonizers and include many putative periodontal pathogens, such as Fusobacterium nucleatum. The red complex (Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola) was considered the climax community and is on the list of putative periodontal pathogens. The 19 major periodontal pathogens tested were expressed at various levels. F. nucleatum was the most abundant species, and the least abundant were Actinomyces viscosus, P. gingivalis, and Aggregatibacter actinomycetemcomitans. The genome counts for Eikenella corrodens, Campylobacter concisus, Campylobacter rectus, T. denticola, and Tannerella forsythensis increased significantly with subject age. The study highlights the observation that bacteria from the yellow complex (Streptococcus spp., S. mitis), the green complex (E. corrodens, Campylobacter gracilis, Capnocytophaga ochracea, Capnocytophaga sputigena, A. actinomycetemcomitans), the purple complex (Veillonella parvula, Actinomyces odontolyticus) and the blue complex (A. viscosus) are correlated. Concerning the orange complex, F. nucleatum is the most abundant species in interdental biofilm. The red complex, which is recognized as the most important pathogen in adult periodontal disease, represents 8.08% of the 19 bacteria analyzed. P. gingivalis was detected in 19% of healthy subjects and represents 0.02% of the interdental biofilm. T. forsythensis and T. denticola (0.02 and 0.04% of the interdental biofilm) were detected in 93 and 49% of healthy subjects, respectively. The effective presence of periodontal pathogens is a strong indicator of the need to develop new methods for disrupting interdental biofilm in daily oral hygiene.