Comparative analysis of expressed sequence tags in resistant and susceptible ecotypes of Arabidopsis thaliana infected with cucumber mosaic virus
Comparative analysis of expressed sequence tags in resistant and susceptible ecotypes of Arabidopsis thaliana infected with cucumber mosaic virus
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DOI：
10.1093/pcp/pch057
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发表时间：
2004-01-01
影响因子：
4.9
通讯作者：
Takahashi, H
中科院分区：
文献类型：
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作者：
Ishihara, T;Sakurai, N;Takahashi, H
拟南芥（Arabidopsis thaliana）生态型哥伦比亚（Col-0）对黄瓜花叶病毒（CMV（Y））黄株系敏感，而生态型C24对CMV（Y）具有抗性。对感染CMV（Y）的Col-0和C24两个生态型的近9,000个表达序列标签的综合分析表明，两个生态型的基因表达模式不同。在CMV（Y）接种后6、12、24和48 h，C24中分别有6、30、85和788个基因的表达发生变化，而在CMV（Y）感染的Col-0中有20、80、53和150个基因。在接种CMV（Y）后12、24和48 h，两种生态型中3、10和55个mRNA的丰度发生了变化。然而，在CMV（Y）接种后6小时，没有基因在两种生态型中被共诱导或共抑制。在CMV（Y）感染的早期阶段，两种生态型之间的基因表达的差异模式表明，在宏阵列可检测的水平上，抗性的细胞反应可能不同于导致易感性的细胞反应。根据感染不同阶段的表达模式，可以使用聚类分析将许多基因的表达分组为簇。约100个编码叶绿体功能相关蛋白的基因被分为簇I和簇4，这两个簇在接种CMV（Y）的C24中具有差异较低的表达。在C24和Col-0接种CMV（Y）的叶片中，内膜系统中编码蛋白质的各种基因的表达属于第2和第4簇。对两种生态型中CMV（Y）基因表达改变的表征将有助于更好地理解病毒与宿主植物之间相容性和不相容性相互作用的分子基础。
Arabidopsis thaliana ecotype Columbia (Col-0) is susceptible to the yellow strain of cucumber mosaic virus [CMV(Y)], whereas ecotype C24 is resistant to CMV(Y). Comprehensive analyses of similar to9,000 expressed sequence tags in ecotypes Col-0 and C24 infected with CMV(Y) suggested that the gene expression patterns in the two ecotypes differed. At 6, 12, 24 and 48 h after CMV(Y) inoculation, the expression of 6, 30, 85 and 788 genes, respectively, had changed in C24, as opposed to 20, 80, 53 and 150 genes in CMV(Y)-infected Col-0. At 12, 24 and 48 h after CMV(Y) inoculation, the abundance of 3, 10 and 55 mRNAs was altered in both ecotypes. However, at 6 h after CMV(Y) inoculation, no genes were co-induced or co-suppressed in both ecotypes. This differential pattern of gene expression between the two ecotypes at an early stage of CMV(Y) infection indicated that the cellular response for resistance may differ from that resulting in susceptibility at the level detectable by the macroarray. According to the expression pattern at various stages of infection, the expression of many genes could be grouped into clusters using cluster analysis. About 100 genes that encode proteins involved in chloroplast function were categorized into clusters I and 4, which had a differentially lower expression in CMV(Y)inoculated C24. The expression of various genes encoding proteins in the endomembrane system belonged to clusters 2 and 4, which were induced in CMV(Y)-inoculated C24 and Col-0 leaves. Characterization of CMV(Y)-altered gene expression in the two ecotypes will contribute to a better understanding of the molecular basis of compatible and incompatible interactions between virus and host plants.