CULTURAL AND ANTIGENIC PROPERTIES OF NEWLY ESTABLISHED CELL STRAINS DERIVED FROM ADENOCARCINOMAS OF HUMAN COLON AND RECTUM
CULTURAL AND ANTIGENIC PROPERTIES OF NEWLY ESTABLISHED CELL STRAINS DERIVED FROM ADENOCARCINOMAS OF HUMAN COLON AND RECTUM
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DOI：
10.1093/jnci/52.4.1101
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发表时间：
1974-01-01
期刊：
影响因子：
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通讯作者：
HARRIS, JA
中科院分区：
文献类型：
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作者：
TOMPKINS, WA;WATRACH, AM;HARRIS, JA
从人下胃肠道的腺癌中建立了两种肿瘤细胞株。这两个菌株，人结肠肿瘤-8(HCT-8)和人直肠肿瘤-18(HRT-18)，都以离散和紧密排列的上皮细胞集落的形式生长，每个细胞都有一个大的核和稀少的细胞质。相当均匀的微绒毛分布在细胞的整个游离面上，通常是平行的，与肠上皮细胞的刷状边缘非常相似。该菌株具有传代时间短、培养效率高等特点。这些细胞在人成纤维细胞上形成集落，但在其他物种的成纤维细胞上不形成集落。细胞遗传学分析显示，HCT-8在体外低、高传代水平上均有48条染色体，其中约90%为额外的A和D染色体。HRT-18也有48条染色体的模式。然而，增加的染色体更加随机，大部分出现在D和F组。HCT-8和HRT-18在体外都产生了高水平的癌胚抗原(CEA)，大多数CEA存在于培养上清液中，与细胞无关。
Two strains of neoplastic cells were established from adenocarcinomas of the human lower gastrointestinal tract. Both strains, human colon tumor-8 (HCT-8) and human rectal tumor-18 (HRT-18), grew as discrete and tightly packed colonies of epithelial cells, each with a large nucleus and scanty cytoplasm. Fairly uniform microvilli, disposed over the entire free surface of the cells, were frequently parallel, closely resembling the brush border of the intestinal epithelial cells. The strains were characterized by a short generation time and high plating efficiency. The cells formed colonies when plated on human fibroblasts but did not form colonies on fibroblasts from other species. Cytogenetic analysis of HCT-8 revealed a mode of 48 chromosomes at both low and high in vitro passage levels, with extra A and D chromosomes in approximately 90%. HRT-18 also had a mode of 48 chromosomes. However, the additional chromosomes were more random, appearing for the most part in groups D and F. Both HCT-8 and HRT-18 yielded high levels of carcinoembryonic antigen (CEA) in vitro, with most CEA in the culture supernatant and not cell associated.