Localization of a Contact Zone between Two Highly Divergent Mitochondrial DNA Lineages of the Brown Bear Ursus arctos in Scandinavia.
Localization of a Contact Zone between Two Highly Divergent Mitochondrial DNA Lineages of the Brown Bear Ursus arctos in Scandinavia.
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斯堪的纳维亚棕熊 Ursus arctos 两个高度分化的线粒体 DNA 谱系之间接触区的定位。
DOI：
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发表时间：
1995
影响因子：
6.3
通讯作者：
A. Bjärvall
中科院分区：
文献类型：
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作者：
P. Taberlet;J. Swenson;F. Sandegren;A. Bjärvall
在欧洲，棕熊（Ursus arctos）由两种不同的线粒体DNA （mtDNA）谱系代表，它们可能在85万年前分化。斯堪的纳维亚半岛已经被来自北方（东部血统）和南方（西部血统）的两种血统的代表殖民，现在熊主要出现在四个主要的地区，称为雌性集中区。为管理目的，确定这两种基因型之间的接触区是很重要的。研究人员利用毛发作为DNA来源，对来自斯堪的纳维亚各地种群的127只棕熊进行了血统分配分析。通过聚合酶链反应扩增一部分mtDNA控制区，并对产物进行测序（14个个体）或用两个诊断限制性内切酶（113个个体）酶切。56只熊和71只熊分别被分配到西部和东部血统。这两种基因型的地理分布使接触区得以精确定位。每个谱系中只有两个雄性跨越了两个谱系之间的边界。我们使用了一岁熊的传播数据来确定潜在的mtDNA渗入是否与熊的传播行为一致。在“错误”区域的雄性与“正确”区域的分散距离都在第95百分位范围内。女性比男性更有爱心，而且没有出现在错误的区域。接触带两侧的两个女性集中区相距134公里。因此，无线电遥测散布距离的结果可以解释雄性出现在错误的遗传区域。在缺乏关于可能的雄性介导的基因流动的信息的情况下，保守的管理方法是将南部和北部三个雌性集中区视为两个不同的保护单元。Localización在接触区，在接触区，在接触区，在接触区，在接触区，在接触区，在接触区，在接触区，在接触区，在接触区，在接触区
In Europe the brown bear (Ursus arctos) is represented by two different mitochondrial DNA (mtDNA) lineages, which probably diverged about 0.85 million years ago. Scandinavia has been colonized by representatives of both lineages, from the north (eastern lineage) and from the south (western lineage), and now bears occur primarily in four main regions called female concentration areas. For management purposes the localization of the contact zone between these two genotypes is important. Using hairs as a source of DNA, 127 individual brown bears from throughout the Scandinavian populations were assayed for lineage assignment. A part of the mtDNA control region was amplified via the polymearase chain reaction, and the product was either sequenced (14 individuals) or digested with two diagnostic restriction endonucleases (113 individuals). Fifty-six and 71 bears were assigned to the western and eastern lineages, respectively. The geographic distribution of the two genotypes allowed precise localization of the contact zone. Only two males from each lineage had crossed the border between the two lineages. We used dispersal data from bears radiomarked as yearlings to determine whether potential mtDNA introgressions agreed with the dispersal behavior of bears. The males in the "wrong" areas were all within the 95th-percentile dispersal distance from the "correct" area. Females were more philopatric than males, and none were found in the wrong areas. The two female concentration areas flanking the contact zone were 134 km apart. Thus, radiotelemetry results on dispersal distances could explain the occurrence of the males in the wrong genetic area. In the absence of information concerning possible male-mediated gene flow, a conservative management approach would be to consider the southern and the three northern female concentration areas as two distinct conservation units. Localización de una zona de contacto entre dos linajes de ADN mitocondrial muy divergentes del oso pardo Ursus arctos en Escandinavia.