Individual neural cell types express immunologically distinct N-CAM forms.
Individual neural cell types express immunologically distinct N-CAM forms.
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DOI：
10.1083/jcb.101.1.36
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发表时间：
1985-07
期刊：
影响因子：
--
通讯作者：
Akeson R
中科院分区：
文献类型：
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作者：
Williams RK;Goridis C;Akeson R
神经细胞黏附分子，或N-cAMs，是一组在脊椎动物神经组织中发现的结构和免疫相关的糖蛋白。成人大脑N-CaM的表观分子量分别为180,000,140,000和120,000。在本文中，我们用单抗3G6.41鉴定了一种免疫上不同的成年大鼠N-CAM形式，并证明这种形式是由一些克隆性神经细胞系选择性表达的。连续的免疫沉淀实验表明，兔抗N-CAM可以从原代培养的溶解的小脑神经元中去除所有与单抗3G6.41反应的18万和14万摩尔的N-CAM分子。然而，单抗3G6.41不能去除所有与兔抗N-CAM反应的N-CAM分子。兔抗N-CAM与大鼠神经细胞系B35、B65和B104、胶质细胞系B12和C6以及L6成肌细胞结合并免疫沉淀形成N-CAM。单抗3G6.41不结合或免疫沉淀B12和B65株的N-CAM，但根据这两个标准确实与其他四株株反应。在原代培养的新生大鼠中，许多表达胶质纤维酸性蛋白的细胞也与单抗3G6.41结合。因此，在一些但不是所有神经细胞、神经胶质细胞和肌肉细胞上发现了一种由单抗3G6.41识别的独特形式的大鼠N-CAM。
The neural cell adhesion molecules, or N-CAMs, are a group of structurally and immunologically related glycoproteins found in vertebrate neural tissues. Adult brain N-CAMs have apparent molecular weights of 180,000, 140,000, and 120,000. In this article we identify, using monoclonal antibody (Mab) 3G6.41, an immunologically distinct adult rat N-CAM form and show that this form is selectively expressed by some clonal neural cell lines. Consecutive immunoprecipitation experiments indicate that rabbit anti-N-CAM can remove from solubilized cerebellar neuron primary cultures all 180,000- and 140,000-mol-wt N- CAM molecules that react with Mab 3G6.41. However Mab 3G6.41 cannot remove all N-CAM molecules that react with rabbit anti-N-CAM. Rabbit anti-N-CAM binds to and immunoprecipitates N-CAM forms from the rat neuronal cell lines B35, B65, and B104, the glial lines B12 and C6, and L6 myoblasts. Mab 3G6.41 does not bind to or immunoprecipitate N-CAM from the B12 and B65 lines but does react with the other four lines by both criteria. Many cells in primary cultures of postnatal rat that express glial fibrillary acidic protein also bind Mab 3G6.41. Thus a unique form of rat N-CAM recognized by Mab 3G6.41 is found on some but not all neuronal, glial, and muscle cells.