Exogenous Melatonin Improves Cold Tolerance of Strawberry (Fragaria x ananassa Duch.) through Modulation of DREB/CBF-COR Pathway and Antioxidant Defense System
Exogenous Melatonin Improves Cold Tolerance of Strawberry (Fragaria x ananassa Duch.) through Modulation of DREB/CBF-COR Pathway and Antioxidant Defense System
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外源褪黑素通过调节 DREB/CBF-COR 通路和抗氧化防御系统提高草莓 (Fragaria à ananassa Duch.) 的耐寒性
DOI：
10.3390/horticulturae8030194
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发表时间：
2022-03-01
期刊：
影响因子：
3.1
通讯作者：
Gu, Xianbin
中科院分区：
文献类型：
--
作者：
Hayat, Faisal;Sun, Zixun;Gu, Xianbin
草莓（Fragaria x ananassa Duch.）因其独特的口感和营养特性而成为世界范围内重要的水果作物。与草莓生产相关的主要风险之一是冷害。近年来，褪黑激素作为一种影响植物生长发育和减少冷胁迫不良后果的多功能信号分子被发现。本研究旨在探讨褪黑素在低温胁迫下草莓植株的防御作用及其与脱落酸（ABA）的潜在相互关系。结果表明，褪黑素通过降低低温胁迫下草莓幼苗丙二醛和过氧化氢含量，提高了草莓幼苗的抗寒性。相反，100 μ M褪黑素处理草莓植株可提高可溶性糖含量，提高冷胁迫下不同抗氧化酶（抗坏血酸过氧化物酶、过氧化氢酶和过氧化物酶）和非酶抗氧化酶（抗坏血酸和谷胱甘肽）活性。此外，外源性褪黑激素刺激了DREB/CBF-COR通路下游基因的表达。有趣的是，ABA处理没有改变DREB/CBF-COR通路的表达。这些发现表明，DREB/CBF-COR途径通过外源褪黑激素的施用赋予草莓幼苗耐冷性。综上所述，我们的研究结果表明，褪黑素（100 μ M）预处理通过增强抗氧化防御潜能和调节DREB/CBF-COR通路来保护草莓植株免受冷胁迫的伤害。
The strawberry (Fragaria x ananassa Duch.) is an important fruit crop cultivated worldwide for its unique taste and nutritional properties. One of the major risks associated with strawberry production is cold damage. Recently, melatonin has emerged as a multifunctional signaling molecule that influences plant growth and development and reduces adverse consequences of cold stress. The present study was conducted to investigate the defensive role of melatonin and its potential interrelation with abscisic acid (ABA) in strawberry plants under cold stress. The results demonstrate that melatonin application conferred improved cold tolerance on strawberry seedlings by reducing malondialdehyde and hydrogen peroxide contents under cold stress. Conversely, pretreatment of strawberry plants with 100 mu M melatonin increased soluble sugar contents and different antioxidant enzyme activities (ascorbate peroxidase, catalase, and peroxidase) and non-enzymatic antioxidant (ascorbate and glutathione) activities under cold stress. Furthermore, exogenous melatonin treatment stimulated the expression of the DREB/CBF-COR pathways' downstream genes. Interestingly, ABA treatment did not change the expression of the DREB/CBF-COR pathway. These findings imply that the DREB/CBF-COR pathway confers cold tolerance on strawberry seedlings through exogenous melatonin application. Taken together, our results reveal that melatonin (100 mu M) pretreatment protects strawberry plants from the damages induced by cold stress through enhanced antioxidant defense potential and modulating the DREB/CBF-COR pathway.