Measurement of Multiple Cardiac Performance Endpoints in Daphnia and Zebrafish by Kymograph
Measurement of Multiple Cardiac Performance Endpoints in Daphnia and Zebrafish by Kymograph
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DOI：
10.3390/inventions6010008
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发表时间：
2021-03-01
期刊：
影响因子：
3.4
通讯作者：
Hsiao, Chung-Der
中科院分区：
文献类型：
--
作者：
Kurnia, Kevin Adi;Saputra, Ferry;Hsiao, Chung-Der
心血管疾病（CVD）是全球头号死亡原因。这种情况导致了对CVD的大量研究，增加了对适合体内研究的动物模型的需求。水蚤和斑马鱼由于其相对透明的身体和易于培养的特性，是心血管研究的良好动物模型。已经开发了几种方法来测量水蚤和斑马鱼的心脏性能。然而，大多数方法只能获得心跳率。其他重要的心脏终点，如每搏输出量、射血分数、缩短分数、心输出量和心跳规律性，必须使用其他程序进行测量。为了克服这一局限性，在这项研究中，我们成功地开发了一个一站式的ImageJ为基础的方法，使用记波宏语言，能够同时获得多个心脏性能终点的第一次。为了验证其实用性，我们在不同的环境温度下孵育大型蚤，并将斑马鱼暴露于阿司咪唑，以检测相应的心脏性能变化。总之，本研究中报告的记波法提供了一种新的、易于使用的、廉价的一站式方法，可获得多个心脏性能终点，具有高准确性和便利性。
Cardiovascular disease (CVD) is the number one cause of death worldwide. This condition resulted in huge research on CVD increasing the need for animal models suitable for in vivo research. Daphnia and zebrafish are good animal models for cardiovascular research due to their relative body transparency and easy culture property. Several methods have been developed to conduct cardiac performance measurement in Daphnia and zebrafish. However, most of the methods are only able to obtain heartbeat rate. The other important cardiac endpoints like stroke volume, ejection fraction, fraction shortening, cardiac output, and heartbeat regularity must use other programs for measurement. To overcome this limitation, in this study, we successfully developed a one-stop ImageJ-based method using kymograph macros language that is able to obtain multiple cardiac performance endpoints simultaneously for the first time. To validate its utility, we incubated Daphnia magna at different ambient temperatures and exposed zebrafish with astemizole to detect the corresponding cardiac performance alterations. In summary, the kymograph method reported in this study provides a new, easy to use, and inexpensive one-stop method obtaining multiple cardiac performance endpoints with high accuracy and convenience.