Biomedical advances from tissue culture
Biomedical advances from tissue culture
复制标题
DOI：
10.1007/s10616-013-9591-1
复制
发表时间：
2013-12-01
期刊：
影响因子：
2.2
通讯作者：
Sato，Gordon H.
中科院分区：
文献类型：
--
作者：
Okamoto，Tetsuji;Sato，J. Denry;Sato，Gordon H.
在组织培养的早期通常观察到的细胞“去分化”是由于成纤维细胞的选择性过度生长，这一证明导致了富集培养技术（动物和培养物交替传代），该技术旨在为功能分化的肿瘤细胞提供选择性优势。这些实验导致衍生出大量的功能分化的克隆株的细胞类型的范围。这些结果产生了这样的假设，即培养中的细胞准确地代表体内细胞，但没有复杂的体内环境。这一概念已得到加强，与功能分化的克隆细胞系，这增加了组织培养研究的潜力相结合的功能定义的培养基的发展。使用经组织学确定的培养基代替补充血清的培养基表明，可以在培养物中发现激素反应和依赖性。癌细胞的激素依赖性的发现导致了针对细胞内信号通路的治疗，而多能细胞的激素反应的发现有助于确定干细胞的潜在应用。在这些和其他方面，组织培养技术将继续有助于解决人类健康问题。
The demonstration that the “dedifferentiation” of cells commonly observed in the early days of tissue culture was due to selective overgrowth of fibroblasts led to enrichment culture techniques (alternate animal and culture passage) designed to give a selective advantage to functionally differentiated tumor cells. These experiments resulted in the derivation of a large number of functionally differentiated clonal strains of a range of cell types. These results gave rise to the hypothesis that cells in culture accurately represent cells in vivo but without the complex in vivo environment. This concept has been strengthened with the development of hormonally defined culture media in combination with functionally differentiated clonal cell lines, which have augmented the potential of tissue culture studies. The use of hormonally defined media in place of serum-supplemented media demonstrates that hormonal responses and dependencies can be discovered in culture. Discoveries of hormonal dependencies of cancer cells has led to therapies targeting intracellular signaling pathways while discoveries of hormonal responses of pluripotent cells are helping to identify the potential application of stem cells. In these and other ways tissue culture technology will continue to contribute to solving problems of human health.