Collagen Nerve Conduits Promote Enhanced Axonal Regeneration, Schwann Cell Association, and Neovascularization Compared to Silicone Conduits
Collagen Nerve Conduits Promote Enhanced Axonal Regeneration, Schwann Cell Association, and Neovascularization Compared to Silicone Conduits
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DOI：
10.1089/ten.tea.2008.0338
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发表时间：
2009-08-01
影响因子：
4.1
通讯作者：
Midha, Rajiv
中科院分区：
文献类型：
--
作者：
Kemp, Stephen W. P.;Syed, M. A. Shahbaz;Midha, Rajiv
引导导管内的外周神经再生涉及再生轴突、许旺细胞（SC）和新血管形成之间的关键联系。然而，目前尚不清楚在非渗透性与半渗透性神经导管中这些因素之间是否存在更大的关联。因此，我们在大鼠坐骨神经的5 mm和10 mm损伤间隙中研究了硅树脂和胶原基神经导管的这种合作。结果表明，在较短的5 mm间隙模型中，与硅胶导管相比，胶原导管促进了增强的轴突和SC再生和缔合。此外，胶原管显示出比硅胶导管增强的新血管形成，表明这三个因素在成功的周围神经再生中密切相关。在10 mm间隙模型中的后期时间点（1个月和2个月分析），与硅胶导管相比，胶原管显示轴突再生、髓鞘形成和血管化增强。这些研究的结果表明，与硅胶导管相比，胶原基导管内的再生电缆更早且更完全地再血管化，这反过来又增强了通过这些神经导管的周围神经再生。
Peripheral nerve regeneration within guidance conduits involves a critical association between regenerating axons, Schwann cells (SCs), and neovascularization. However, it is currently unknown if there is a greater association between these factors in nonpermeable versus semipermeable nerve guide conduits. We therefore examined this collaboration in both silicone- and collagen-based nerve conduits in both 5- and 10-mm-injury gaps in rat sciatic nerves. Results indicate that collagen conduits promoted enhanced axonal and SC regeneration and association when compared to silicone conduits in the shorter 5- mm-gap model. In addition, collagen tubes displayed enhanced neovascularization over silicone conduits, suggesting that these three factors are intimately related in successful peripheral nerve regeneration. At later time points (1- and 2-month analysis) in a 10-mm-gap model, collagen tubes displayed enhanced axonal regeneration, myelination, and vascularization when compared to silicone- based conduits. Results from these studies suggest that regenerating cables within collagen-based conduits are revascularized earlier and more completely, which in turn enhances peripheral nerve regeneration through these nerve guides as compared to silicone conduits.