Elevated expression of activation molecules by decidual lymphocytes in women suffering spontaneous early pregnancy loss
Elevated expression of activation molecules by decidual lymphocytes in women suffering spontaneous early pregnancy loss
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DOI：
10.1093/humrep/14.5.1194
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发表时间：
1999-05-01
影响因子：
6.1
通讯作者：
Bulmer, JN
中科院分区：
文献类型：
--
作者：
Vassiliadou, N;Searle, RF;Bulmer, JN
本研究的目的是调查，量化和比较的活化标志物的表达蜕膜白细胞在散发性自发性早孕流产和明显正常的第一个三个月的人妊娠。蜕膜取自18例治疗性流产和20例8-12周胎龄的散发性自然流产。冰冻切片用抗生物素蛋白-生物素复合物-过氧化物酶法标记，使用对CD 45、CD 56、CD 3、人类白细胞抗原（HLA）DR、CD 69、CD 25和极晚期抗原（VLA）1特异的单克隆抗体，对阳性细胞进行定量，并使用Mann-Whitney统计检验分析结果。与治疗性流产蜕膜相比，自然流产蜕膜中CD 69阳性和CD 25阳性细胞数量显著增加，约50%的自然流产妇女蜕膜中HLA DR阳性细胞数量也显著增加，免疫组化双重标记结果显示，自然流产蜕膜中CD 25和CD 69阳性细胞均为CD 3-T细胞。阳性T细胞而不是CD 56阳性颗粒淋巴细胞。子宫内膜免疫功能紊乱可能是散发性自然流产的原因之一。
The purpose of this study was to investigate, quantify and compare the expression of activation markers by decidual leukocytes in sporadic spontaneous early pregnancy loss and apparently normal first trimester human pregnancy. Decidua was obtained from 18 therapeutic abortions and 20 sporadic spontaneous abortions at 8-12 weeks gestational age. Cryostat sections were labelled by the avidin-biotin complex-peroxidase method using monoclonal antibodies specific for CD45, CD56, CD3, human leukocyte antigen (HLA) DR, CD69, CD25 and very late antigen (VLA)1, Positive cells were quantified and the results were analysed using the Mann-Whitney statistical test. Significantly increased numbers of CD69-positive and CD25-positive cells were detected in spontaneous abortion decidua, when compared with therapeutic abortion decidua, Approximately 50% of women experiencing spontaneous miscarriage also contained significantly elevated numbers of HLA DR-positive cells within decidua, Double immunohistochemical labelling studies demonstrated that the CD25-positive and CD69-positive cells in spontaneous abortion decidua were CD3-positive T cells rather than CD56-positive granulated lymphocytes. Immunological dysfunction within endometrium may account for a proportion of sporadic spontaneous abortions.