PATHOGENESIS OF THE LUPUS DERMATOSES IN AUTOIMMUNE MICE .15. CHARACTERIZATION OF CUTANEOUS INFILTRATES IN MRL/LPR MICE MONITORED FROM ONSET TO THE FULL DEVELOPMENT OF LUPUS ERYTHEMATOSUS-LIKE SKIN-LESIONS
PATHOGENESIS OF THE LUPUS DERMATOSES IN AUTOIMMUNE MICE .15. CHARACTERIZATION OF CUTANEOUS INFILTRATES IN MRL/LPR MICE MONITORED FROM ONSET TO THE FULL DEVELOPMENT OF LUPUS ERYTHEMATOSUS-LIKE SKIN-LESIONS
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DOI：
10.1111/1523-1747.ep12470176
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发表时间：
1991-04-01
影响因子：
6.5
通讯作者：
IMAMURA, S
中科院分区：
文献类型：
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作者：
KANAUCHI, H;FURUKAWA, F;IMAMURA, S
皮肤是红斑狼疮（lupus erythematosus，LE）的主要损害部位，但这种损害是否是由单核细胞浸润引起的以及哪些免疫活性细胞在皮肤LE的发生发展中起主要作用仍有争议。 为了更好地表征免疫活性细胞的作用，我们对MRL/Mp-lpr/lpr（MRL/lpr）小鼠LE样皮肤病变中的这些细胞进行了免疫组织化学检查。 对60只雌性MRL/lpr小鼠的皮肤病变从发病到完全发育进行监测。 对每个阶段的皮肤标本进行表皮Ia+朗格汉斯细胞（Ia+-LC）、Thy-1+表皮树枝状细胞（Thy-1+DEC）以及单核细胞浸润表型染色。 皮损中Ia ~+-LC和Thy-1 ~+DEC的数量在后期明显减少。 Ia ~+-LC早期在病灶中心部位明显增多，晚期在病灶周边部位明显增多。 真皮浸润早期以L3 T4+细胞为主，L3 T4/Lyt-2比值较高。 随着临床分期的进展，真皮浸润组织中L3 T4/Lyt-2比值逐渐降低，而淋巴结中Thy-1.2/Lyt-2比值则相反。 表皮下真皮浸润以L3 T4+细胞为主，Ia+-LC数量增多。 皮肤LE的小鼠模型的免疫组化分析显示，随着皮肤病变的发展，免疫活性细胞群的变化，我们得出结论，Ia+-LC和Thy-1+DEC，以及L3 T4+和Lyt-2+细胞，可能在皮肤病变的发展中发挥致病作用。
The skin is a primary site injured in lupus erythematosus (LE), but it is still controversial whether the injury is due to cells of the mononuclear infiltrate and which immunocompetent cells play the major role in the development of cutaneous LE. To better characterize the role of immunocompetent cells, we performed an immunohistochemical examination of these cells in LE-like skin lesions in MRL/Mp-lpr/lpr (MRL/lpr) mice. Skin lesions in 60 female MRL/lpr mice were monitored from onset to full development. Skin specimens from each stage were stained for epidermal Ia+ Langerhans cells (Ia+-LC), for Thy-1+ dendritic epidermal cells (Thy-1+DEC), and for the phenotype of the mononuclear cell infiltrates. The numbers of Ia+-LC and Thy-1+DEC were decreased markedly in the skin lesions at the later stage. However, the numbers of Ia+-LC were increased significantly in the central portion of lesions at an early stage and in the peripheral portion of lesions later. L3T4+ cells were predominant, and the L3T4/Lyt-2 ratio was high in dermal infiltrates at an early stage. With advancing stage, the L3T4/Lyt-2 ratio gradually decreased in dermal infiltrates, whereas the Thy-1.2/Lyt-2 ratio in lymph nodes was reversed. L3T4+ cells were especially predominant in dermal infiltrates under the epidermis with increased numbers of Ia+-LC. This immunohistochemical analysis of a mouse model of cutaneous LE revealed changes in immunocompetent cell populations with the evolution of skin lesions, and we conclude that Ia+-LC and Thy-1+DEC, as well as L3T4+ and Lyt-2+ cells, may play pathogenic roles in the development of skin lesions.