Possibility of real-time measurement of an airborne Cryptomeria japonica pollen allergen based on the principle of surface plasmon resonance
Possibility of real-time measurement of an airborne Cryptomeria japonica pollen allergen based on the principle of surface plasmon resonance
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基于表面等离子体共振原理实时测量空气中柳杉花粉过敏原的可能性
DOI：
10.1023/a:1013002001583
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发表时间：
2001
期刊：
影响因子：
2
通讯作者：
H. Nitta
中科院分区：
文献类型：
--
作者：
Yuichi Takahashi;T. Ohashi;T. Nagoya;M. Sakaguchi;H. Yasueda;H. Nitta
为了建立花粉变应原浓度的实时检测系统，我们以日本柳杉主要变应原之一Cry j 1为例建立了该系统。该系统包括：用虚拟撞击器或旋风采样器收集空气中的花粉变应原，用含有0.125M NH 4 HCO 3的10 mM HEPES缓冲液提取Cry j 1，最后用BIACORE 3000系统实时测量Cry j 1。该方法的灵敏度为5 ng/ml，每次测定需0.1ml样品体积和至少500 pg的Cry j 1。空气中Cry j 1的定量可在采样后30 min测定，即15 min提取，10 min分离，5 min测定。
In order to establish a real-timemeasurement system for the concentration ofairborne pollen allergens, we examined Cry j 1,one of the major allergens ofCryptomeriajaponicapollen, as an example to establishthe system. The feasible system consisted of:collection of airborne pollen allergens usingthe Virtual Impactor or Cyclone sampler,extraction of Cry j 1 using 10mM HEPES buffercontaining 0.125M NH4HCO3andfinally, real-time measurement of Cry j 1 usingthe BIACORE 3000 system. The sensitivity of thesystem was 5 ng/ml, and 0.1 ml sample volumeand at least 500 pg of Cry j 1 were requiredfor each measurement. Quantification of Cry j 1in the air can be determined 30 min aftercollection, i.e. 15 min for extraction, 10 minfor separation from particulate matters and 5min for the measurement.