Expression of glioma-associated antigens in pediatric brain stem and non-brain stem gliomas
Expression of glioma-associated antigens in pediatric brain stem and non-brain stem gliomas
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DOI：
10.1007/s11060-008-9566-9
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发表时间：
2008-07-01
影响因子：
3.9
通讯作者：
Pollack, Ian F.
中科院分区：
文献类型：
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作者：
Okada, Hideho;Low, Keri L.;Pollack, Ian F.
我们研究了三种胶质瘤相关抗原（GAA）在儿童脑干胶质瘤（BSG）和非脑干胶质瘤（NBSG）病例中的蛋白表达，以期其可能用于免疫治疗。采用免疫组织化学方法检测15例BSG和12例NBSG石蜡包埋组织中EphA 2、IL-13 R α 2和Survivin的表达。15个BSG中的13个和所有12个NBSG表达至少一种GAA; 7个BSG和9个NBSG以高于非肿瘤性脑的水平表达至少两种GAA。肿瘤分级与GAA表达水平之间无相关性。虽然许多病例显示GAA在整个标本中弥漫表达，但在少数病例中观察到部分或斑片状表达，这表明需要在免疫治疗中靶向多种GAA。这些结果表明EphA 2、IL-13 Ra 2和Survivin是开发儿童胶质瘤疫苗策略的合适靶点。
We investigated the protein expression of three glioma-associated antigens (GAAs) in pediatric brain stem glioma (BSG) and non-brain stem glioma (NBSG) cases with a view to their possible use in immunotherapy. Expression of EphA2, IL-13R alpha 2 and Survivin were studied by immunohistochemistry on paraffin-embedded tissues using a series of 15 BSG cases and 12 NBSG cases. Thirteen of 15 BSGs and all 12 NBSGs expressed at least one of GAAs; and 7 BSGs and 9 NBSGs expressed at least two of these GAAs at higher levels than non-neoplastic brain. There was no association between the tumor grade and levels of GAA expression. Although many cases demonstrated diffuse expression of GAAs throughout specimens, partial or patchy expression was noted in a small number of cases, suggesting a need for targeting multiple GAAs in immunotherapy. These results suggest that EphA2, IL-13Ralpha2 and Survivin are suitable targets for developing vaccine strategies for pediatric glioma.