Celecoxib inhibits Helicobacter pylori colonization-related factors.
Celecoxib inhibits Helicobacter pylori colonization-related factors.
复制标题
DOI：
10.3748/wjg.v16.i7.846
复制
发表时间：
2010-02
影响因子：
4.3
通讯作者：
Jing Wang;Weihong Wang;Jiang‐Dong Li;Fang-Xun Liu
中科院分区：
文献类型：
--
作者：
Jing Wang;Weihong Wang;Jiang‐Dong Li;Fang-Xun Liu
目的探讨选择性考克斯-2抑制剂塞来昔布对幽门螺杆菌（H. pylori）定植相关因素及其机制。方法用塞来昔布与H. pylori菌株26695在透射电子显微镜下观察。用刺琼脂运动试验检测鞭毛运动。坚持H。pylori对AGS细胞的杀伤作用采用酶联免疫吸附试验。实时荧光定量PCR检测鞭毛基因（flaA、flaB）、尿素酶基因（ureA、ureB）和粘附素基因（babA、sabA、alpA、alpB、hpaA、hopZ）的mRNA表达水平。结果细菌细胞壁分离、不完整，细胞质疏松、不对称，H。在塞来昔布存在下观察到幽门螺杆菌。当H. pylori菌株在塞来昔布存在下孵育，它们的鞭毛运动和对AGS细胞的粘附被抑制。塞来昔布可上调ureA、ureB、babA、sabA、alpA、alpB、hpaA、hopZ的表达，下调flaA、flaB的表达。结论塞来昔布抑制H. pylori感染AGS细胞，破坏其正常结构。
AIM To investigate the effect of celecoxib, a selective COX-2 inhibitor, on Helicobacter pylori (H. pylori) colonization-related factors and its mechanism. METHODS After co-incubation with celecoxib, morphology of H. pylori strain 26695 was observed under a transmission electron microscope. Flagella motility was assessed by stab agar motility test. Adherence of H. pylori to AGS cells was determined by enzyme linked immunosorbent assay. Levels of mRNA expression in flagellar genes (flaA, flaB), urease genes (ureA, ureB) and adhesin genes (babA, sabA, alpA, alpB, hpaA, hopZ) were measured by real-time polymerase chain reaction. RESULTS Separation and non-integrity of bacterial cell wall, rarefaction and asymmetry of cytoplasm, and even lysis of H. pylori were observed in the presence of celecoxib. When H. pylori strains were incubated in the presence of celecoxib, their flagellar motility and adherence to AGS cells were inhibited. The expression of ureA, ureB, babA, sabA, alpA, alpB, hpaA, hopZ was up-regulated while the expression of flaA, flaB was down-regulated in the presence of celecoxib. CONCLUSION Celecoxib inhibits flagellar motility and adherence of H. pylori to AGS cells, and destructs their normal structure in vitro.