EPSTEIN-BARR-VIRUS DNA IN CEREBROSPINAL-FLUID FROM PATIENTS WITH AIDS-RELATED PRIMARY LYMPHOMA OF THE CENTRAL-NERVOUS-SYSTEM
EPSTEIN-BARR-VIRUS DNA IN CEREBROSPINAL-FLUID FROM PATIENTS WITH AIDS-RELATED PRIMARY LYMPHOMA OF THE CENTRAL-NERVOUS-SYSTEM
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DOI：
10.1016/0140-6736(93)92814-a
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发表时间：
1993-08-14
期刊：
影响因子：
168.9
通讯作者：
LINDE, A
中科院分区：
文献类型：
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作者：
CINQUE, P;BRYTTING, M;LINDE, A
爱泼斯坦-巴尔病毒(EBV)经常与艾滋病相关的中枢神经系统(CNS)原发淋巴瘤有关。为探讨脑脊液(CSF)中EB病毒DNA作为肿瘤标志物的可行性，采用套式聚合酶链式反应(PCR)技术对85例HIV感染和神经系统疾病患者临终前180天内采集的脑脊液标本进行了EBV检测。组织学上发现17例原发性中枢神经系统淋巴瘤，16例组织中EBV原位杂交法阳性。17例原发性中枢神经系统淋巴瘤患者脑脊液中均检出EBV DNA。在68例无组织学可检出淋巴瘤的HIV感染者中，有1例在脑脊液中发现EBV DNA。脑脊液中EB病毒DNA的聚合酶链式反应对艾滋病相关的原发中枢神经系统淋巴瘤的敏感性为100%，特异性为98.5%，可作为一种诊断肿瘤标志物。
Epstein-Barr virus (EBV) is constantly associated with AIDS-related primary lymphomas of the central nervous system (CNS). To assess whether EBV DNA in cerebrospinal fluid (CSF) could be used as a tumour marker, CSF samples that had been taken within 180 days before death from 85 patients with HIV infection and neurological disorders at necropsy were examined retrospectively by nested polymerase chain reaction (PCR) for EBV. Histologically evident primary CNS lymphomas were found in 17 patients, and EBV was shown in tissue by in-situ hybridisation in 16 of the 16 cases examined. All 17 patients with primary CNS lymphoma had EBV DNA in CSF. EBV DNA was found in CSF from 1 of 68 HIV-infected patients without histologically detectable lymphoma at necropsy. PCR for EBV DNA in CSF was 100% sensitive and 98.5% specific for AIDS-associated primary CNS lymphoma, and may be useful as a diagnostic tumour marker.