Expression of type I mGluRs predicts plasticity in the hippocampal stratum radiatum interneurons.
Expression of type I mGluRs predicts plasticity in the hippocampal stratum radiatum interneurons.
复制标题
I 型 mGluR 的表达预测海马放射层中间神经元的可塑性。
DOI：
10.1016/j.neulet.2019.134472
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发表时间：
2019
影响因子：
2.5
通讯作者：
Edwards,JeffreyG
中科院分区：
文献类型：
--
作者：
Nufer,TeresaM;Merrill,Collin;Friend,Lindsey;Hopkins,Zach;Boyce,Zach;Edwards,JeffreyG
海马CA 1区锥体细胞突触之间突触强度的变化是记忆获得的部分原因。这种可塑性由放射层中的前馈抑制性中间神经元调制。虽然放射性中间神经元经历长期抑郁（LTD），短期抑郁（STD）或缺乏可塑性，但尚不清楚可塑性是否与特定的中间神经元亚型相关。使用全细胞电生理学和实时定量PCR，我们的特点是可塑性表达不同的中间神经元亚型。我们首先分析了钙结合蛋白和胆囊收缩素mRNA的表达模式，以确定细胞亚型。然后，我们评估了内源性大麻素（eCB）生物合成酶mRNA的表达，包括二酰甘油脂肪酶α，N-酰基-磷脂酰乙醇胺磷脂酶D和12-脂氧合酶，以及通常介导可塑性的代谢型谷氨酸受体。表现出LTD的神经元倾向于共表达至少一种eCB生物合成酶和代谢型谷氨酸受体5（mGluR 5）的mRNA。相反，mGluR 5不表达的神经元表现出STD或没有可塑性。显示STD的神经元倾向于表达至少一种eCB生物合成酶和mGluR 1的mRNA，但不表达mGluR 5。这表明放射层中间神经元的可塑性可以基于I型mGluR表达来预测。
Changes in synaptic strength between hippocampal CA1 pyramidal cell synapses are partly responsible for memory acquisition. This plasticity is modulated by feedforward inhibitory interneurons in the stratum radiatum. While radiatum interneurons experience either long-term depression (LTD), short-term depression (STD), or lack plasticity, it is unclear whether plasticity correlates to specific interneuron subtypes. Using whole-cell electrophysiology and real-time quantitative PCR, we characterized the plasticity expressed by different interneuron subtypes. We first analyzed calcium binding proteins and cholecystokinin mRNA expression patterns to determine cell subtype. We then assessed endocannabinoid (eCB) biosynthetic enzyme mRNA expression, including diacylglycerol lipase α, N-acyl-phosphatidylethanolamine phospholipase D, and 12-lipoxygenase, and metabotropic glutamate receptors that often mediate plasticity. Neurons exhibiting LTD tended to co-express mRNA for at least one eCB biosynthetic enzyme and the metabotropic glutamate receptor 5 (mGluR5). Conversely, mGluR5 was not expressed by neurons exhibiting STD or no plasticity. Neurons that exhibited STD tended to express mRNA for at least one eCB biosynthetic enzyme and mGluR1, but not mGluR5. This suggests that plasticity of stratum radiatum interneurons could be predicted based on type I mGluR expression.