Odor-induced currents in Xenopus olfactory receptor cells measured with perforated-patch recording.
Odor-induced currents in Xenopus olfactory receptor cells measured with perforated-patch recording.
复制标题
用穿孔贴片记录测量非洲爪蟾嗅觉受体细胞中的气味诱导电流。
DOI：
10.1152/jn.1995.74.1.479
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Ache,BW
中科院分区：
文献类型：
--
作者：
Zhainazarov,AB;Ache,BW
1.气味诱发电流记录在非洲爪蟾嗅觉受体神经元（ORN）的使用常规的，以及制霉菌素和短杆菌肽穿孔，全细胞记录。在传统的，但不是在穿孔，全细胞记录的气味诱发的电流迅速下降。所有三种类型的记录给出了相似的值的振幅，潜伏期，时间到峰值，恢复时间，和反转电位的气味诱发电流。2.次级Cl电流占气味诱发电流的很大一部分（55-65%）。用短杆菌肽穿孔法测得的EC 1为-2.3 +/- 5.0（SE）mV，但不改变[Cl-]i，表明这些神经元保持高[Cl-]i，次级Cl电流在嗅觉传导中起兴奋作用。
1. Odor-evoked currents were recorded in Xenopus laevis olfactory receptor neurons (ORNs) by the use of conventional, as well as nystatin and gramicidin-perforated, whole cell recording. The odor-evoked current ran down quickly in conventional, but not in perforated, whole cell recording. All three types of recording gave similar values for the amplitude, latency, time-to-peak, recovery time, and reversal potential of the odor-evoked current. 2. A secondary Cl current comprised a significant part of the odor-evoked current (55-65%). ECl measured by gramicidin perforation, which does not alter [Cl-]i, was -2.3 +/- 5.0 (SE) mV, indicating that these neurons maintain a high [Cl-]i and that the secondary Cl current plays an excitatory role in olfactory transduction.