Comparative genetic mapping in Boechera stricta, a close relative of Arabidopsis
Comparative genetic mapping in Boechera stricta, a close relative of Arabidopsis
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DOI：
10.1104/pp.107.096685
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发表时间：
2007-05-01
期刊：
影响因子：
7.4
通讯作者：
Mitchell-Olds, Thomas
中科院分区：
文献类型：
--
作者：
Schranz, M. Eric;Windsor, Aaron J.;Mitchell-Olds, Thomas
被子植物科拟南芥科包含研究模型拟南芥（Arabidopsis thaliana）和农业属芸苔属（Brassica）。拟南芥科的比较基因组学主要集中在拟南芥和感兴趣的物种之间的直接比较。然而，减少基因组大小和染色体数目（n = 5）的拟南芥复杂的比较。拟南芥显示广泛的基因组和染色体改组相比，它的近亲拟南芥和羽衣甘蓝rubella，都与n = 8。为了促进比较基因组学在拟南芥科，我们最近概述了一个系统的24个保守的染色体块的基础上，他们的位置在祖先核型的n 5 - 8，而不是他们的位置在拟南芥。在这份报告中，我们使用这个系统作为一个工具，以了解基因组结构和进化Boechera stricta（n = 7）。B。stricta是一种二倍体，有性，高度自交的物种，主要分布在北美西部的山区。我们已经创建了B的F-2遗传图谱。stricta基于192个个体在196个微卫星和候选基因位点上得分。单核苷酸多态性基因分型的94个位点同时使用Illumina珠阵列。图谱总长度为725.8 cM，平均标记间距为3.9 cM。不存在大于19.3 cM的间隙。B的染色体数目由n 5 8减少到n 5 7以及其他基因组变化。stricta可能涉及臂间倒位、染色体融合和两个相互易位，这些都很容易用基因组块观察到。我们的遗传图谱将有助于分析Boechera生态相关的数量变化。
The angiosperm family Brassicaceae contains both the research model Arabidopsis ( Arabidopsis thaliana) and the agricultural genus Brassica. Comparative genomics in the Brassicaceae has largely focused on direct comparisons between Arabidopsis and the species of interest. However, the reduced genome size and chromosome number (n = 5) of Arabidopsis complicates comparisons. Arabidopsis shows extensive genome and chromosome reshuffling compared to its close relatives Arabidopsis lyrata and Capsella rubella, both with n = 8. To facilitate comparative genomics across the Brassicaceae we recently outlined a system of 24 conserved chromosomal blocks based on their positions in an ancestral karyotype of n 5 8, rather than by their position in Arabidopsis. In this report we use this system as a tool to understand genome structure and evolution in Boechera stricta (n = 7). B. stricta is a diploid, sexual, and highly self-fertilizing species occurring in mostly montane regions of western North America. We have created an F-2 genetic map of B. stricta based on 192 individuals scored at 196 microsatellite and candidate gene loci. Single-nucleotide polymorphism genotyping of 94 of the loci was done simultaneously using an Illumina bead array. The total map length is 725.8 cM, with an average marker spacing of 3.9 cM. There are no gaps greater than 19.3 cM. The chromosomal reduction from n 5 8 to n 5 7 and other genomic changes in B. stricta likely involved a pericentric inversion, a chromosomal fusion, and two reciprocal translocations that are easily visualized using the genomic blocks. Our genetic map will facilitate the analysis of ecologically relevant quantitative variation in Boechera.