A human chromosome 22 fosmid resource: mapping and analysis of 96 clones.
A human chromosome 22 fosmid resource: mapping and analysis of 96 clones.
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人类 22 号染色体 fosmid 资源：96 个克隆的定位和分析。
DOI：
10.1006/geno.1996.0246
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Simon,MI
中科院分区：
文献类型：
--
作者：
Birren,BW;Tachi-iri,Y;Kim,UJ;Nguyen,M;Shizuya,H;Korenberg,JR;Simon,MI
我们已经创建了一个22号染色体的资源，由96个独特的，特征良好的Fossils组成。Fosmid载体可以在基于大肠杆菌F因子的单拷贝载体中高效克隆平均40kb的DNA片段，我们发现来自人22号染色体的Fosmid克隆显示出显著的稳定性，并且在基因组分析中有广泛的应用。这96个克隆已被定位的FISH，使用高分辨率荧光显带和荧光定位技术，它们在染色体上的位置与它们的内容的一些共同的重复序列元件。我们通过使用NotI、MluI、SacII和BssHII酶的限制性分析，鉴定了一组可能含有基因的克隆。这些细胞遗传学锚定克隆占染色体的近7%，对于检测染色体重排、用于基因分离和作为物理作图的框架具有直接价值。
We have created a resource for chromosome 22 consisting of 96 unique, well-characterized Fosmids. The Fosmid vector permits efficient cloning of DNA fragments averaging 40 kb in a single-copy vector based on the F factor ofEscherichia coli.We have found that Fosmid clones from human chromosome 22 show remarkable stability and are useful for a wide variety of applications in genome analysis. These 96 clones have been localized by FISH, using high-resolution fluorescent banding and multicolor mapping techniques, and their position on the chromosome was correlated with their content of a number of common repeated sequence elements. We identified a subset of clones likely to contain genes by restriction analysis using the enzymesNotI,MluI,SacII, andBssHII. This collection of cytogenetically anchored clones, representing nearly 7% of the chromosome, is of immediate value for detecting chromosomal rearrangements, for use in gene isolation, and as a framework for physical mapping.