Full-Malaria/Parasites and Full-Arthropods: databases of full-length cDNAs of parasites and arthropods, update 2009
Full-Malaria/Parasites and Full-Arthropods: databases of full-length cDNAs of parasites and arthropods, update 2009
复制标题
DOI：
10.1093/nar/gkn856
复制
发表时间：
2009-01-01
影响因子：
14.9
通讯作者：
Watanabe, Junichi
中科院分区：
文献类型：
--
作者：
Wakaguri, Hiroyuki;Suzuki, Yutaka;Watanabe, Junichi
Full-Malaria/Parasites是一个基于我们原始的全长cDNA序列和物理克隆资源的转录组研究尖端复合体和其他寄生虫的数据库。在这次更新中，数据库已经扩展到包含来自6个顶端复合体寄生虫和680万个转录起始点(TS)的14 818个非冗余全长cDNA克隆的整个序列的幕府序列，这两个克隆都是通过使用寡核苷酸封顶方法和Illumina GA测序仪的新协议产生的。前者应该是对表达基因进行准确注释的最终数据，而后者应该是用于超深层表达分析的有用数据。此外，我们还推出了全长节肢动物数据库，这是一个具有重要医学意义的节肢动物的全长cdna数据库。全节肢动物在传播疟疾的按蚊、传播锥虫病的采采蝇和引起过敏性皮炎和哮喘的尘螨中包含50 343个一遍序列、10 399个猎枪完整序列和2240万个TSS标签。通过提供顶丛寄生虫及其媒介中最大的整合全长cDNA数据资源，全疟原虫/寄生虫和全节肢动物应该有助于对抗寄生虫病。全疟疾/寄生虫和全节肢动物可从http://fullmal.hgc.jp/.获取
Full-Malaria/Parasites is a database for transcriptome studies of apicomplexa and other parasites, which is based on our original full-length cDNA sequences and physical cDNA clone resources. In this update, the database has been expanded to contain the shogun sequencing for the entire sequences of 14 818 non-redundant full-length cDNA clones from six apicomplexa parasites and 6.8 million of transcription start sites (TSS), both of which had been produced by novel protocols using the oligo-capping method and the Illumina GA sequencer. The former should be the ultimate data for exact annotation of the expressed genes, while the latter should be useful for ultra-deep expression analysis. Furthermore, we have launched Full-Arthropods, a full-length cDNA database for arthropods of medical importance. Full-Arthropods contains 50 343 one-pass sequences, 10 399 shotgun complete sequences and 22.4 million TSS tags in anopheles mosquitoes that transmit malaria, tsetse flies that transmit trypanosomiasis and dust mites that cause allergic dermatitis and bronchial asthma. By providing the largest integrated full-length cDNA data resources in the apicomplexa parasites as well as their vectors, Full-Malaria/Parasites and Full-Arthropods should help combat parasitic diseases. Full-Malaria/Parasites and Full-Arthropods are accessible from http://fullmal.hgc.jp/.