Characterisation and epitope mapping of neutralising monoclonal antibodies to A24 Cruzeiro strain of FMDV
Characterisation and epitope mapping of neutralising monoclonal antibodies to A24 Cruzeiro strain of FMDV
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DOI：
10.1016/j.vetmic.2010.11.003
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发表时间：
2011-04-21
影响因子：
3.3
通讯作者：
Paton, D. J.
中科院分区：
文献类型：
--
作者：
Mahapatra, M.;Seki, C.;Paton, D. J.
对7种抗口蹄疫病毒A(24) Cruzeiro的中和性单克隆抗体（mab）进行了鉴定，发现了3个反应性基团。gr - 1识别线性表位，而Gr-II是构象依赖和胰蛋白酶不敏感的。Gr-III也是构象依赖的，但对胰蛋白酶敏感。Mar（抗单抗中和）突变体只能针对gr - 1和Gr-III单抗产生。通过与亲本病毒的衣壳序列比较，发现VP1的G-H环在141,143和147位点发生了变化。类似地，Gr-III mar突变体在VP1的148位显示出从高度保守的甘氨酸到色氨酸的变化，以及VP1， VP2和VP4的n端另外三个变化。VP1的148位残基位于“RGD”后的+2位置，相当于血清型O病毒中单克隆抗体识别位点5所识别的位置。这个位点可能是由于G-H环与不同结构蛋白中的其他残基相互作用而形成的。(C) 2010 Elsevier B.V.版权所有
Characterisation of seven neutralising monoclonal antibodies (mAbs) produced against foot-and-mouth disease virus A(24) Cruzeiro revealed three reactivity groups. Gr-I recognised linear epitopes where as Gr-II was conformation-dependent and trypsin-insensitive. The Gr-III was also conformation-dependent, but trypsin-sensitive. Mar (mAb neutralisation resistant)-mutants could only be produced against Gr-I and Gr-III mAbs. Capsid sequence comparison of Gr-I mar-mutants with parent virus revealed changes in the G-H loop of VP1 at positions 141,143 and 147. Similarly, a Gr-III mar-mutant showed a change from a highly conserved glycine to a tryptophan at position 148 of VP1 along with three additional changes at the N-terminus of VP1, VP2 and VP4. This residue at 148 of VP1 is located at +2 position after "RGD" and is equivalent to the position identified by the mAb recognising site 5 in serotype O viruses. This site is probably formed because of the interaction of the G-H loop with other residues in different structural proteins. (C) 2010 Elsevier B.V. All rights reserved.