Parallel Evolution of Sperm Hyper-Activation Ca2+ Channels.
Parallel Evolution of Sperm Hyper-Activation Ca2+ Channels.
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DOI：
10.1093/gbe/evx131
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发表时间：
2017-07-01
影响因子：
3.3
通讯作者：
Phadnis N
中科院分区：
文献类型：
--
作者：
Cooper JC;Phadnis N
精子过度激活是精子行为的一种戏剧性变化，成熟精子在与卵子的竞争中突然进入最后冲刺。在包括人类在内的许多后生动物中，精子超激活的机制是由Ca2+通过CatSper离子通道进入精子鞭毛的震荡组成的。令人惊讶的是，所有九个CatSper基因都在几个动物谱系中独立地消失了。在果蝇中，精子超激活是通过多囊肾病2 (pkd2) Ca2+通道的选择进行的。灵长类动物的CatSpers和果蝇的pkd2之间的相似之处提供了一个独特的机会来研究精子超激活机制在两个独立的、非同源的钙通道中的分子进化，这两个钙通道被5亿年的分化分开。在这里，我们使用全面的系统基因组方法来研究这些精子超激活通道的选择压力。首先，我们发现在灵长类动物中，整个CatSper复合体在反复的正选择下迅速进化。其次，我们发现pkd2在果蝇中具有相似的适应性进化模式。第三，我们发现pkd2的适应性进化是由它在精子超激活中的作用驱动的。这些选择模式表明，精子超激活机制的进化是由调节通道活性的拮抗配体的性冲突驱动的。总之，我们的研究结果为快速进化的生殖蛋白类增加了精子超激活通道，并为两性操纵精子行为的机制提供了见解。
Sperm hyper-activation is a dramatic change in sperm behavior where mature sperm burst into a final sprint in the race to the egg. The mechanism of sperm hyper-activation in many metazoans, including humans, consists of a jolt of Ca2+ into the sperm flagellum via CatSper ion channels. Surprisingly, all nine CatSper genes have been independently lost in several animal lineages. In Drosophila, sperm hyper-activation is performed through the cooption of the polycystic kidney disease 2 (pkd2) Ca2+ channel. The parallels between CatSpers in primates and pkd2 in Drosophila provide a unique opportunity to examine the molecular evolution of the sperm hyper-activation machinery in two independent, nonhomologous calcium channels separated by > 500 million years of divergence. Here, we use a comprehensive phylogenomic approach to investigate the selective pressures on these sperm hyper-activation channels. First, we find that the entire CatSper complex evolves rapidly under recurrent positive selection in primates. Second, we find that pkd2 has parallel patterns of adaptive evolution in Drosophila. Third, we show that this adaptive evolution of pkd2 is driven by its role in sperm hyper-activation. These patterns of selection suggest that the evolution of the sperm hyper-activation machinery is driven by sexual conflict with antagonistic ligands that modulate channel activity. Together, our results add sperm hyper-activation channels to the class of fast evolving reproductive proteins and provide insights into the mechanisms used by the sexes to manipulate sperm behavior.
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DOI：
10.1073/pnas.0605795103
发表时间：
2006-10-10
影响因子：
11.1
作者：
Gomendio, Montserrat;Martin-Coello, Juan;Roldan, Eduardo R. S.
通讯作者：
Roldan, Eduardo R. S.
影响因子：
9.8
作者：
Hamm, David;Mautz, Brian S.;Swanson, Willie J.
通讯作者：
Swanson, Willie J.
种群基因组学：果蝇果蝇中多态性和差异的全基因组分析。
影响因子：
9.8
作者：
Begun, David J.;Holloway, Alisha K.;Stevens, Kristian;Hillier, LaDeana W.;Poh, Yu-Ping;Hahn, Matthew W.;Nista, Phillip M.;Jones, Corbin D.;Kern, Andrew D.;Dewey, Colin N.;Pachter, Lior;Myers, Eugene;Langley, Charles H.
通讯作者：
Langley, Charles H.
影响因子：
16.6
作者：
通讯作者：
--
影响因子：
4.8
作者：
Cai, ZQ;Maeda, Y;Somlo, S
通讯作者：
Somlo, S