A Minimized Valveless Electromagnetic Micropump for Microfluidic Actuation on Organ Chips
A Minimized Valveless Electromagnetic Micropump for Microfluidic Actuation on Organ Chips
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DOI：
10.1016/j.sna.2019.111704
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发表时间：
2020-01-01
影响因子：
4.6
通讯作者：
Sun, Wei
中科院分区：
文献类型：
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作者：
Mi, Shengli;Pu, Haitao;Sun, Wei
近年来，器官芯片已成为生理和病理研究以及药物筛选的理想选择。为了更真实地模拟体内生理环境，通常需要微泵作为动力源来实现器官芯片的动态培养。在这项研究中，我们提出了一种可用于器官芯片的无阀电磁微泵。流体流动是由PDMS膜通过变化磁场的振动驱动的。这种微型泵使用喷嘴/扩散器结构代替阀门，简化了结构。通过减小线圈和磁铁的体积，电磁微泵的尺寸被最小化，从而可以集成在微流控芯片上。微型泵和芯片可以通过使用小型信号产生模块和干电池为线圈提供方波来便携封装，从而减少对外部设备的依赖。此外，多个电磁微泵可以集成在一个芯片上。这些微泵可以串联或并联，可以实现复杂的流动条件，可以更真实地模拟不同类型器官芯片中的生理流体流动。通过测量流量来表征微泵的驱动性能。我们在芯片上建立了肝癌和乳腺癌模型的动态共培养，介质由电磁微泵驱动。分析细胞活力、白蛋白和白细胞介素-6。结果表明，与静态对照组相比，由电磁微泵驱动的动态共培养有利于细胞的生长和功能。(C) 2019 Elsevier B.V.版权所有
In recent years, organ chips have become an ideal alternative for physiological and pathological studies as well as drug screening. To more realistically simulate the in vivo physiological environment, micropumps are often needed as power sources to achieve dynamic culture in organ chips. In this study, we present a valveless electromagnetic micropump that can be used in organ chips. Fluid flow is actuated by the vibration of a PDMS membrane through a varying magnetic field. This micropump uses a nozzle/diffuser structure instead of valves, which simplifies the structure. By reducing the volume of the coil and magnet, the size of the electromagnetic micropump is minimized so that it can be integrated on a microfluidic chip. The micropump and the chip can be portably packaged by using a small signal generation module and a dry battery to supply the coil with a square wave, reducing the dependence on external devices. In addition, multiple electromagnetic micropumps can be integrated on a single chip. These micropumps can be connected in series or in parallel, which can achieve complicated flow conditions and can simulate physiological fluid flow more realistically in different types of organ chips. The flow rate was measured to characterize the actuating performance of the micropump. We established the dynamic coculture of a liver and breast cancer model on the chip, with medium actuated by the electromagnetic micropump. Cell viability, albumin and IL-6 were analyzed. The results indicated that dynamic coculture, which was actuated by the electromagnetic micropump, was beneficial for cell growth and function compared with the static control group. (C) 2019 Elsevier B.V. All rights reserved.