Identification of Root-Secreted Compounds Involved in the Communication Between Cucumber, the Beneficial Bacillus amyloliquefaciens, and the Soil-Borne Pathogen Fusarium oxysporum
Identification of Root-Secreted Compounds Involved in the Communication Between Cucumber, the Beneficial Bacillus amyloliquefaciens, and the Soil-Borne Pathogen Fusarium oxysporum
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参与黄瓜、有益解淀粉芽孢杆菌和土传病原体尖孢镰刀菌之间通讯的根部分泌化合物的鉴定
DOI：
10.1094/mpmi-07-16-0131-r
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发表时间：
2017-01-01
影响因子：
3.5
通讯作者：
Zhang, Ruifu
中科院分区：
文献类型：
--
作者：
Liu, Yunpeng;Chen, Lin;Zhang, Ruifu
促进植物生长的根际细菌(PGPR)的定植是发挥其对植物有益作用的关键。根分泌物是影响PGPR和土传病原菌在根系内定植的主要因素。然而，PGPR、植物根系和土传病原菌之间的三方相互作用还知之甚少。我们筛选了根分泌物，以寻找在根际中调节三方相互作用的信号。在分根系统中，我们发现PGPR菌株SQR9在黄瓜根上的定植显著高于接种SQR9或土传尖孢镰刀菌。Cucumerum的根部定植，而尖孢霉根定殖。预先接种SQR9或尖孢霉F.sp.可降低黄瓜产量。黄瓜。预先接种SQR9或尖孢霉的黄瓜根分泌物对黄瓜进行了成分分析，共鉴定出109个化合物。相关分析表明，有8个化合物与SQR9或尖孢霉F。黄瓜。在用这些化学物质进行定殖实验后，棉子糖和色氨酸对尖孢镰刀菌的根部定殖有积极的影响。分别为黄瓜和SQR9。这些结果表明，黄瓜根部受到尖孢霉的侵染。黄瓜或SQR9增加根中色氨酸的分泌，以加强SQR9的进一步定植。相反，这些定植的黄瓜根减少棉子糖的分泌，从而抑制尖孢霉的根部定殖。黄瓜。
Colonization of plant growth promoting rhizobacteria (PGPR) is critical for exerting their beneficial effects on the plant. Root exudation is a major factor influencing the colonization of both PGPR and soil-borne pathogens within the root system. However, the tripartite interaction of PGPR, plant roots, and soil-borne pathogens is poorly understood. We screened root exudates for signals that mediate tripartite interactions in the rhizosphere. In a split-root system, we found that root coloni-zation of PGPR strain Bacillus amyloliquefaciens SQR9 on cucumber root was significantly enhanced by preinoculation with SQR9 or the soil-borne pathogen Fusarium oxysporum f. sp. cucumerinum, whereas root colonization of F oxysporum f. sp. cucumerinum was reduced upon preinoculation with SQR9 or F oxysporum f. sp. cucumerinum. Root exudates from cucum-bers preinoculated with SQR9 or F oxysporum f. sp. cucumer-inum were analyzed and 109 compounds were identified. Correlation analysis highlighted eight compounds that sig-nificantly correlated with root colonization of SQR9 or F oxysporum f. sp. cucumerinum. After performing colonization experiments with these chemicals, raffinose and tryptophan were shown to positively affect the root colonization of F. oxysporum f. sp. cucumerinum and SQR9, respectively. These results indicate that cucumber roots colonized by F oxysporum f. sp. cucumerinum or SQR9 increase root secretion of tryptophan to strengthen further colonization of SQR9. In contrast, these colonized cucumber roots reduce raffinose secretion to inhibit root colonization of F oxysporum f. sp. cucumerinum.