Antibacterial photodynamic therapy mediated by 5-aminolevulinic acid on methicillin-resistant Staphylococcus aureus
Antibacterial photodynamic therapy mediated by 5-aminolevulinic acid on methicillin-resistant Staphylococcus aureus
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DOI：
10.1016/j.pdpdt.2019.09.008
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发表时间：
2019-12-01
影响因子：
3.3
通讯作者：
Wang, Hongwei
中科院分区：
文献类型：
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作者：
Huan, Jianhua;Guo, Mingquan;Wang, Hongwei
耐甲氧西林金黄色葡萄球菌（MRSA）等耐药菌的出现给临床治疗带来了很大困难。抗菌光动力疗法（aPDT）是一种新型的非抗生素治疗策略，可用于治疗多种耐药菌。然而，很少有研究5-氨基乙酰丙酸介导的光动力疗法（ALA-PDT）对MRSA的抗菌机制。本研究观察了ALA-PDT对MRSA的杀菌作用。我们发现当ALA浓度为0.05 mM时，ALA-PDT具有最强的杀菌效果，并且aPDT的杀菌活性随着光剂量的增加而增加。MRSA在0.05 mM和384 Jcm（-2）时几乎完全消除。此外，还使用LIVE/DEAD（R）BacLighe（TM）细菌活力试剂盒和电子显微镜在荧光显微镜下观察杀菌形态。同时发现ALA-PDT对蛋白质和DNA有损伤作用。最后，通过qRT-PCR分析，MRSA特异性基因nuc的转录水平在ALA-PDT处理后降低了0.74倍（P < 0.05）。结果提示ALA-PDT可通过损伤细胞膜、细胞质、蛋白质和核酸等途径有效抑制MRSA。
The emergence of drug-resistant bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), has brought great difficulties to clinical treatment. Antibacterial photodynamic therapy (aPDT) is a new non-antibiotic treatment strategy for a variety of drug-resistant bacteria. However, there are few studies on the antimicrobial mechanism of a PDT mediated by 5-aminolevulinic acid (ALA-PDT) for MRSA. In this study, we observed the bactericidal effect of ALA-PDT on MRSA. We found that ALA-PDT had the strongest bactericidal effect when ALA was at 0.05 mM, and the bactericidal activity of aPDT increased with the increase of light dose. MRSA was almost completely eliminated at 0.05 mM and 384 Jcm(-2). In addition, the bactericidal morphology was also observed under a fluorescence microscope using a LIVE/DEAD (R) BacLighe (TM) Bacterial Viability Kit and an electron microscope. It was also found that proteins and DNA were damaged by ALA-PDT. Finally, the transcription level of the specific gene of MRSA, nuc, was decreased by 0.74-fold (P < 0.05) after ALA-PDT treatment by qRT-PCR analysis. The findings suggest that ALA-PDT can effectively inhibit MRSA by damaging cell membrane, cytoplasm, proteins and nucleic acid.