Application of spontaneously immortalized odontoblast cells in tooth regeneration
Application of spontaneously immortalized odontoblast cells in tooth regeneration
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DOI：
10.1016/j.bbrc.2009.02.025
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发表时间：
2009-03-27
影响因子：
3.1
通讯作者：
Sugiyama, Toshihiro
中科院分区：
文献类型：
--
作者：
Arany, Szilvia;Kawagoe, Masami;Sugiyama, Toshihiro
在这里，我们报告的第一次尝试使用一个自发永生间充质细胞系的生物工程牙齿。为了评估该细胞系的成牙本质潜能，将成牙本质细胞谱系细胞（OLC）与从E14.5小鼠分离的感受态牙上皮重新结合。采用一种新的三维器官胚培养方法进行体外培养。此外，将重组体移植到宿主动物的肾包膜下2周。在三分之一的病例中，移植物发育成牙齿组织。OLC衍生的GFP阳性细胞可以通过免疫组化在矿化牙胚中鉴定。OLC能够进行细胞间和细胞-基质间的通讯，从而最终分化为功能性成牙本质细胞。总之，我们成功地在牙齿再生实验中利用OLC进行牙齿间充质替代。因此，我们的自发转化细胞系证明了其在未来复杂的牙齿发育和生物工程研究中的潜力。(C)2009 Elsevier Inc. All rights reserved.
Here, we report on the first attempt to bioengineer tooth using a spontaneously immortalized mesenchymal cell line. To assess the odontogenic potential of this cell line, odontoblast-lineage cells (OLC) were re-associated with competent dental epithelium isolated from E14.5 mice. A novel three-dimensional organ germ culture method was applied to nurture the constructs in vitro. Additionally, recombinants were transplanted under the kidney capsule in host animals for 2 weeks. Transplants developed into tooth tissues in one-third of the cases. OLC-derived GFP-positive cells could be identified in mineralizing tooth germs by immunohistochemistry. OLCs were capable of intercellular and cell-matrix communication, thus they eventually differentiated into functional odontoblasts. In summary, we managed to utilize OLCs for dental mesenchyme substitution in tooth regeneration experiments. Therefore, our spontaneously transformed cell line proved its potential for future complex, tooth developmental and bioengineering studies. (C) 2009 Elsevier Inc. All rights reserved.