The transcripts of SFRP1, CEP63 and EIF4G2 genes are frequently downregulated in transitional cell carcinomas of the bladder
The transcripts of SFRP1, CEP63 and EIF4G2 genes are frequently downregulated in transitional cell carcinomas of the bladder
复制标题
DOI：
10.1159/000090984
复制
发表时间：
2005-01-01
期刊：
影响因子：
3.5
通讯作者：
Nagai, MA
中科院分区：
文献类型：
--
作者：
Buim, ME;Soares, FA;Nagai, MA
目的：本研究的目的是寻找可能与浅表性和浸润性膀胱癌表型相关的差异表达基因。方法：采用差异显示逆转录聚合酶链式反应(DDRT-PCR)方法，比较正常膀胱组织和4组膀胱移行细胞癌组织中不同临床分期和分级的表达情况。结果：我们能够识别出72个不同的转录本，其中57个(79%)与已知基因同源，12个(17%)与假设蛋白同源，3个(4%)与人类表达序列标签同源。在这些差异表达的基因中，SFRP1、CEP63和EIF4G2在一系列50例移行细胞癌中被定量RT-PCR进一步验证。总体而言，在所分析的膀胱肿瘤中，这三个基因的转录产物显示下调。DDRT-PCR结果显示，与正常膀胱组织相比，90%(45/50)的膀胱肿瘤组织中SFRP1的转录水平下调。尽管EIF4G2和CEP63转录本显示出三种不同的表达模式，但在所分析的案例中，约有50%的表达下调。此外，CEP63和EIF4G2基因转录下调与侵袭性肿瘤有关。结论：使用DDRT-PCR分析比较不同亚型膀胱肿瘤的表达模式，使我们能够确定与不同细胞通路有关的大量基因，当上调或下调时，这些基因可能在膀胱肿瘤的发生过程中发挥作用。版权所有(C)2005 S.Karger AG，巴塞尔。
Objective: The aim of the present study was to identify differentially expressed genes that might be associated with the phenotype of superficial and invasive bladder cancer. Methods: Differential display reverse transcriptase PCR ( DDRT-PCR) was used to compare the expression pattern between normal bladder tissue and 4 groups of transitional cell carcinomas of the bladder regarding clinical stage and grade. Results: We were able to identify 72 different transcripts, of which 57 (79%) showed homology to known genes, 12 (17%) to hypothetical proteins and 3 (4%) to human expressed sequence tags. Among the differentially expressed genes, SFRP1, CEP63 and EIF4G2 were further validated by quantitative RTPCR in a series of 50 transitional cell carcinomas. Overall, the transcripts of these three genes were shown to be downregulated in the bladder tumors analyzed. In accordance with the DDRT-PCR results, the SFRP1 transcripts were shown to be downregulated in 90% (45/50) of the bladder tumors as compared with the normal bladder tissue. Although EIF4G2 and CEP63 transcripts exhibited three different expression patterns, downregulation was found in about 50% of the cases analyzed. In addition, downregulation of both CEP63 and EIF4G2 gene transcription was associated with invasive tumors. Conclusion: The use of DDRT-PCR analysis to compare expression patterns among different subgroups of bladder tumors allowed us to identify a significant number of genes implicated in different cellular pathways that, when up- or downregulated, might play a role in the tumorigenic process of the bladder. Copyright (C) 2005 S. Karger AG, Basel.